The exploitation of self-assembled systems to improve the solubility of drugs is getting more and more attention. Among the different types of self-assembled biomaterials, peptides and in particular peptides containing non-coded amino acids (NCAPs) are promising because their use opens the door to more stable materials inducing increased stability to proteolysis. New classes of NCAP, Ac-Ala-X-Ala-Aib- AlaCONH2 (X ¼ alpha-aminoisobutyric acid (Aib) or X ¼ cyclopentane amino acid (Ac5c)) have been prepared and the correlation between the different secondary peptide structure and solvent (i.e. CD3CN, CD3OH, H2O/D2O) verified by NMR. Furthermore, the formation of a nanocolloidal system in water was deeply studied by DLS and the morphology of the obtained spherical aggregates with nanometric dimensions was assessed by TEM. Aib containing pentapeptide was selected for greater ease of synthesis. Its ability to encapsulate curcumin, as a model insoluble drug molecule, was investigated using fluorescence emission and confocal microscopy analyses. Two different approaches were used to study the interaction between curcumin and peptide aggregates. In the first approach peptide aggregates were formed in the presence of curcumin, while in the second approach curcumin was added to the already formed peptide aggregates. We succeeded in our challenge by using the second approach and 53.8% of added curcumin had been encapsulated.
Self-assembled hydrophobic Ala-Aib peptide encapsulating curcumin: a convenient system for water insoluble drugs / S. Locarno, S. Argentiere, A. Ruffoni, D. Maggioni, R. Soave, R. Bucci, E. Erba, C. Lenardi, M.L. Gelmi, F. Clerici. - In: RSC ADVANCES. - ISSN 2046-2069. - 10:17(2020 Mar 09), pp. 9964-9975.
|Titolo:||Self-assembled hydrophobic Ala-Aib peptide encapsulating curcumin: a convenient system for water insoluble drugs|
LOCARNO, SILVIA ALICE (Primo)
ARGENTIERE, SIMONA (Secondo)
GELMI, MARIA LUISA (Penultimo)
CLERICI, FRANCESCA (Ultimo) (Corresponding)
|Settore Scientifico Disciplinare:||Settore CHIM/06 - Chimica Organica|
Settore CHIM/02 - Chimica Fisica
Settore CHIM/03 - Chimica Generale e Inorganica
|Data di pubblicazione:||9-mar-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1039/C9RA10981A|
|Appare nelle tipologie:||01 - Articolo su periodico|