The ability to produce cell patterning through precise surface engineering has stimulated the development of cellular bioassays that offer new insights on the mechanisms of cell adhesion, proliferation, differentiation and molecular signaling pathways. Here we describe a simple micropatterning technique combining supersonic cluster beam deposition of nanostructured titania films on bovine serum albumin functionalized substrates. A standard lift-off process enables us to generate complementary micropatterns of hydrophobic bovine serum albumin (cell-repellent) and hydrophilic nanostructured TiO(x) (cell-adhesive). We demonstrate the selective PC12 cell adhesion and growth on biocompatible nanostructured TiO(x). We also observed that these functional micropatterned substrates promote a considerable enhancement of cell attachment and proliferation.

A simple lift-off-based patterning method for micro- and nanostructuring of functional substrates for cell culture / A.V. Singh, C. Lenardi, L. Gailite, A. Gianfelice, P. Milani. - In: JOURNAL OF MICROMECHANICS AND MICROENGINEERING. - ISSN 0960-1317. - 19:11(2009 Nov), pp. 115028.1-115028.8.

A simple lift-off-based patterning method for micro- and nanostructuring of functional substrates for cell culture

A.V. Singh
Primo
;
C. Lenardi
Secondo
;
L. Gailite;A. Gianfelice
Penultimo
;
P. Milani
2009

Abstract

The ability to produce cell patterning through precise surface engineering has stimulated the development of cellular bioassays that offer new insights on the mechanisms of cell adhesion, proliferation, differentiation and molecular signaling pathways. Here we describe a simple micropatterning technique combining supersonic cluster beam deposition of nanostructured titania films on bovine serum albumin functionalized substrates. A standard lift-off process enables us to generate complementary micropatterns of hydrophobic bovine serum albumin (cell-repellent) and hydrophilic nanostructured TiO(x) (cell-adhesive). We demonstrate the selective PC12 cell adhesion and growth on biocompatible nanostructured TiO(x). We also observed that these functional micropatterned substrates promote a considerable enhancement of cell attachment and proliferation.
titanium surfaces; in-vitro; TIO2; phosphorylation; differentiation; proliferation; topography; attachment; adhesion; respond
Settore FIS/03 - Fisica della Materia
nov-2009
Article (author)
File in questo prodotto:
File Dimensione Formato  
pdf.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 1.77 MB
Formato Adobe PDF
1.77 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/71922
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 22
  • ???jsp.display-item.citation.isi??? 20
social impact