Cigarette smoke (CS) is one of the most important preventable risk factors for the development of respiratory diseases, cardiovascular diseases, stroke, and various types of cancer. Due to its high intracellular concentration and central role in maintaining the cellular redox state, glutathione (GSH) is one of the key players in several enzymatic and non-enzymatic reactions necessary for protecting cells against CS-induced oxidative stress. A plethora of in vitro cell models have been used over the years to assess the effects of CS on intracellular GSH and its disulphide forms, i.e. glutathione disulphide (GSSG) and S-glutathionylated proteins. In this review, we described the effects of cell exposure to CS on cellular GSH and formation of its oxidized forms and adducts (GSH-conjugates). We also discussed the limitations and relevance of in vitro cell models of exposure to CS and critically assessed the congruence between smokers and in vitro cell models. What emerges clearly is that results obtained in vitro should be interpreted with extreme caution, bearing in mind the limitations of the specific cell model used. Despite this, in vitro cell models remain important tools in the assessment of CS-induced oxidative damage.

Cigarette smoke and glutathione: Focus on in vitro cell models / I. Dalle-Donne, M.L. Garavaglia, G. Colombo, E. Astori, M.C. Lionetti, C.A.M. La Porta, A. Santucci, R. Rossi, D. Giustarini, A. Milzani. - In: TOXICOLOGY IN VITRO. - ISSN 0887-2333. - 65(2020 Jun), pp. 104818.1-104818.14. [10.1016/j.tiv.2020.104818]

Cigarette smoke and glutathione: Focus on in vitro cell models

I. Dalle-Donne
Primo
;
M.L. Garavaglia
Secondo
;
G. Colombo;E. Astori;M.C. Lionetti;C.A.M. La Porta;A. Milzani
Ultimo
2020-06

Abstract

Cigarette smoke (CS) is one of the most important preventable risk factors for the development of respiratory diseases, cardiovascular diseases, stroke, and various types of cancer. Due to its high intracellular concentration and central role in maintaining the cellular redox state, glutathione (GSH) is one of the key players in several enzymatic and non-enzymatic reactions necessary for protecting cells against CS-induced oxidative stress. A plethora of in vitro cell models have been used over the years to assess the effects of CS on intracellular GSH and its disulphide forms, i.e. glutathione disulphide (GSSG) and S-glutathionylated proteins. In this review, we described the effects of cell exposure to CS on cellular GSH and formation of its oxidized forms and adducts (GSH-conjugates). We also discussed the limitations and relevance of in vitro cell models of exposure to CS and critically assessed the congruence between smokers and in vitro cell models. What emerges clearly is that results obtained in vitro should be interpreted with extreme caution, bearing in mind the limitations of the specific cell model used. Despite this, in vitro cell models remain important tools in the assessment of CS-induced oxidative damage.
Cigarette smoke; Glutathione; in vitro cell models; Oxidative stress; Smokers; Oxidative damage
Settore MED/04 - Patologia Generale
Settore BIO/06 - Anatomia Comparata e Citologia
PIANO DI SOSTEGNO ALLA RICERCA 2015-2017 - LINEA 2 "DOTAZIONE ANNUALE PER ATTIVITA' ISTITUZIONALE"
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/719206
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