Genomes represent the starting point of genetic studies. Since the discovery of DNA structure, scientists have devoted great efforts to determine their sequence in an exact way. In this review we provide a comprehensive historical background of the improvements in DNA sequencing technologies that have accompanied the major milestones in genome sequencing and assembly, ranging from early sequencing methods to Next-Generation Sequencing platforms. We then focus on the advantages and challenges of the current technologies and approaches, collectively known as Third Generation Sequencing. As these technical advancements have been accompanied by progress in analytical methods, we also review the bioinformatic tools currently employed in de novo genome assembly, as well as some applications of Third Generation Sequencing technologies and high-quality reference genomes.
Long walk to genomics : history and current approaches to genome sequencing and assembly / A.M. Giani, G.R. Gallo, L. Gianfranceschi, G. Formenti. - In: COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL. - ISSN 2001-0370. - 18(2020), pp. 9-19.
|Titolo:||Long walk to genomics : history and current approaches to genome sequencing and assembly|
GIANI, ALICE MARIA (Primo) (Corresponding)
FORMENTI, GIULIO PAOLO (Ultimo)
|Parole Chiave:||Bioinformatics; Genome assembly; Next-generation; Reference; Sequencing; Third-generation|
|Settore Scientifico Disciplinare:||Settore BIO/18 - Genetica|
Settore BIO/11 - Biologia Molecolare
|Data di pubblicazione:||2020|
|Data ahead of print / Data di stampa:||17-nov-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.csbj.2019.11.002|
|Appare nelle tipologie:||01 - Articolo su periodico|