Wound healing is a complex process regulated by multiple signals and consisting of several phases known as haemostasis, inflammation, proliferation, and remodelling. Keratinocytes, endothelial cells, macrophages, and fibroblasts are the major cell populations involved in wound healing process. Hypoxia plays a critical role in this process since cells sense and respond to hypoxic conditions by changing gene expression. This study assessed the in vitro expression of 77 genes involved in angiogenesis, metabolism, cell growth, proliferation and apoptosis in human keratinocytes (HaCaT), microvascular endothelial cells (HMEC-1), differentiated macrophages (THP-1), and dermal fibroblasts (HDF). Results indicated that the gene expression profiles induced by hypoxia were cell-type specific. In HMEC-1 and differentiated THP-1, most of the genes modulated by hypoxia encode proteins involved in angiogenesis or belonging to cytokines and growth factors. In HaCaT and HDF, hypoxia mainly affected the expression of genes encoding proteins involved in cell metabolism. This work can help to enlarge the current knowledge about the mechanisms through which a hypoxic environment influences wound healing processes at the molecular level.

Effect of hypoxia on gene expression in cell populations involved in wound healing / S. D'Alessandro, A. Magnavacca, F. Perego, M. Fumagalli, E. Sangiovanni, M. Prato, M. Dell'Agli, N. Basilico. - In: BIOMED RESEARCH INTERNATIONAL. - ISSN 2314-6133. - 2019(2019 Aug), pp. 2626374.1-2626374.20. [10.1155/2019/2626374]

Effect of hypoxia on gene expression in cell populations involved in wound healing

S. D'Alessandro
Co-primo
;
A. Magnavacca
Co-primo
;
F. Perego;M. Fumagalli;E. Sangiovanni;M. Dell'Agli
;
N. Basilico
2019

Abstract

Wound healing is a complex process regulated by multiple signals and consisting of several phases known as haemostasis, inflammation, proliferation, and remodelling. Keratinocytes, endothelial cells, macrophages, and fibroblasts are the major cell populations involved in wound healing process. Hypoxia plays a critical role in this process since cells sense and respond to hypoxic conditions by changing gene expression. This study assessed the in vitro expression of 77 genes involved in angiogenesis, metabolism, cell growth, proliferation and apoptosis in human keratinocytes (HaCaT), microvascular endothelial cells (HMEC-1), differentiated macrophages (THP-1), and dermal fibroblasts (HDF). Results indicated that the gene expression profiles induced by hypoxia were cell-type specific. In HMEC-1 and differentiated THP-1, most of the genes modulated by hypoxia encode proteins involved in angiogenesis or belonging to cytokines and growth factors. In HaCaT and HDF, hypoxia mainly affected the expression of genes encoding proteins involved in cell metabolism. This work can help to enlarge the current knowledge about the mechanisms through which a hypoxic environment influences wound healing processes at the molecular level.
Settore MED/04 - Patologia Generale
Settore BIO/14 - Farmacologia
Settore BIO/10 - Biochimica
   Hypoxia and chronic wounds in the elderly: a multidisciplinary approach from pathogenetic mechanisms to nonconventional nanotherapies
   HyWoNNa
   FONDAZIONE CARIPLO
   2015-0550
ago-2019
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/706202
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