The effects of intracerebroventricular (icv) or subcutaneous (sc) hexarelin (Hexa) administration, against gastric ulcers induced by ethanol (50%, 1 ml/rat/os) or Indomethacin (20 mg/kg/os) were examined in conscious rats. Hexa at 1 nmol/rat, icv or 10 nmol/kg, sc reduced ethanol-induced ulcers by 47% and 32% respectively. Hexa, but not ghrelin significantly worsened (+40%) Indomethacin-induced ulcers when injected sc. Hexa-gastroprotection against ethanol-induced ulcers was removed by the GHS-R antagonist (D-Lys3)-GRPR-6 and by the inhibitor of NO-synthase (NOS) Nomega-nitro-L-arginine methyl ester. Semiquantitative RT-PCR assay of gastric NOS mRNA isoforms revealed that the reduction in iNOS-derived NO and the increase of constitutive-derived NO are relevant for the gastroprotection of Hexa against ethanol-induced gastric damage.
|Titolo:||Effects of hexarelin against acid-indipendent and acid-dependent ulcerogens in the rat|
|Autori interni:||SIBILIA, VALERIA (Primo)|
GUIDOBONO CAVALCHINI, FRANCESCA (Penultimo)
NETTI, CARMELA (Ultimo)
|Parole Chiave:||Gastric ulcer; Ghrelin; GHS-R antagonist; Hexarelin; Nitric oxide synthase|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2004|
|Digital Object Identifier (DOI):||10.1016/j.peptides.2004.08.001|
|Appare nelle tipologie:||01 - Articolo su periodico|
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