Coagulase Negative Staphylococci (CoNS) are becoming increasingly recognized as an important cause of human and animal infections. Notwithstanding their clinical relevance, annotation of genes potentially involved in pathogenicity and/or antibiotic resistance in the CoNS species Staphylococcus arlettae (SAR) is currently very limited. In the current work we describe the genome of a novel methicillin resistant isolate of SAR, which we named Bari, and present a comprehensive analysis of predicted antibiotic resistance profiles and virulence determinants for all the 22 currently available SAR genomes. By comparing predicted antibiotic resistance and virulence-associated genes with those obtained from a manual selection of 148 bacterial strains belonging to 14 different species of staphylococci and to two “outgroup” species, Bacillus subtilis (BS) and Macrococcus caseoliticus (MC), we derived some interesting observations concerning the types and number of antibiotic resistance-related and virulence-like genes in SAR. Interestingly, almost 50% of the putative antibiotic resistance determinants identified in this work, which include the clinically relevant mec, van, and cls genes, were shared among all the SAR strains herein considered (Bari included). Moreover, comparison of predicted antibiotic resistance profiles suggest that SAR is closely related to well-known pathogenic Staphylococcus species, such as Staphylococcus aureus (SA) and Staphylococcus epidermidis (SE). A similar analysis of predicted virulence factors, revealed that several genes associated with pathogenesis (including, for example, ica, nuc, and ssp), which are commonly found in the genomes of pathogenic staphylococci such as Staphylococcus haemolyticus (SH) and Staphylococcus saprophyticus (SS), are observed also in the SAR strains for which a genomic sequence is available. All in all, we believe that the analyses presented in the current study, by providing a consistent and comprehensive annotation of virulence and antibiotic resistance-related genes in SAR, can constitute a valuable resource for the study of molecular mechanisms of opportunistic pathogenicity in this species.

Staphylococcus arlettae genomics : novel insights on candidate antibiotic resistance and virulence genes in an emerging opportunistic pathogen / A. Lavecchia, M. Chiara, C. De Virgilio, C. Manzari, R. Monno, A. De Carlo, C. Pazzani, D. Horner, G. Pesole, A. Placido. - In: MICROORGANISMS. - ISSN 2076-2607. - 7:11(2019 Nov), pp. 580.1-580.14. [10.3390/microorganisms7110580]

Staphylococcus arlettae genomics : novel insights on candidate antibiotic resistance and virulence genes in an emerging opportunistic pathogen

M. Chiara;D. Horner;
2019

Abstract

Coagulase Negative Staphylococci (CoNS) are becoming increasingly recognized as an important cause of human and animal infections. Notwithstanding their clinical relevance, annotation of genes potentially involved in pathogenicity and/or antibiotic resistance in the CoNS species Staphylococcus arlettae (SAR) is currently very limited. In the current work we describe the genome of a novel methicillin resistant isolate of SAR, which we named Bari, and present a comprehensive analysis of predicted antibiotic resistance profiles and virulence determinants for all the 22 currently available SAR genomes. By comparing predicted antibiotic resistance and virulence-associated genes with those obtained from a manual selection of 148 bacterial strains belonging to 14 different species of staphylococci and to two “outgroup” species, Bacillus subtilis (BS) and Macrococcus caseoliticus (MC), we derived some interesting observations concerning the types and number of antibiotic resistance-related and virulence-like genes in SAR. Interestingly, almost 50% of the putative antibiotic resistance determinants identified in this work, which include the clinically relevant mec, van, and cls genes, were shared among all the SAR strains herein considered (Bari included). Moreover, comparison of predicted antibiotic resistance profiles suggest that SAR is closely related to well-known pathogenic Staphylococcus species, such as Staphylococcus aureus (SA) and Staphylococcus epidermidis (SE). A similar analysis of predicted virulence factors, revealed that several genes associated with pathogenesis (including, for example, ica, nuc, and ssp), which are commonly found in the genomes of pathogenic staphylococci such as Staphylococcus haemolyticus (SH) and Staphylococcus saprophyticus (SS), are observed also in the SAR strains for which a genomic sequence is available. All in all, we believe that the analyses presented in the current study, by providing a consistent and comprehensive annotation of virulence and antibiotic resistance-related genes in SAR, can constitute a valuable resource for the study of molecular mechanisms of opportunistic pathogenicity in this species.
No
English
Antibiotic resistance; Coagulase negative staphylococci; Draft genome; Genomics; NGS sequencing; Staphylococcus arlettae; Virulence
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Pubblicazione scientifica
   Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea
   INMARE
   EUROPEAN COMMISSION
   H2020
   634486
nov-2019
MDPI
7
11
580
1
14
14
Pubblicato
Periodico con rilevanza internazionale
scopus
Aderisco
info:eu-repo/semantics/article
Staphylococcus arlettae genomics : novel insights on candidate antibiotic resistance and virulence genes in an emerging opportunistic pathogen / A. Lavecchia, M. Chiara, C. De Virgilio, C. Manzari, R. Monno, A. De Carlo, C. Pazzani, D. Horner, G. Pesole, A. Placido. - In: MICROORGANISMS. - ISSN 2076-2607. - 7:11(2019 Nov), pp. 580.1-580.14. [10.3390/microorganisms7110580]
open
Prodotti della ricerca::01 - Articolo su periodico
10
262
Article (author)
no
A. Lavecchia, M. Chiara, C. De Virgilio, C. Manzari, R. Monno, A. De Carlo, C. Pazzani, D. Horner, G. Pesole, A. Placido
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/698037
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