Background: We have previously shown that the sustained expression of MARK4L transcripts in glioma and neural progenitors (NHNPs) declines after exposure to antisense MARK4L oligonucleotides in glioblastoma cell lines. Array-CGH confirmed the genomic duplication of MARK4L identified by FISH in a glioblastoma cell line. This background together with literature data on the exogenous association of MARK4 with interphase centrosome prompted us to investigate the sub-cellular localization of the endogenous MARK4L protein aiming at achieving insights on its possible role in the pathomechanisms of glioma. Methods: Immunodetection was carried out to validate the specificity of MARK4L antibody in gliomas and NHNPs. Mass spectrometry was applied for MARK4L protein identification in a representative glioblastoma cell line. Combined biochemical fractionation and immunodetection analyses were performed to confirm the sub-cellular localization of MARK4L achieved by immunofluorescence in glioma cell lines. Results: By assigning MARK4L protein within the band immunoprecipitated by the specific antibody we validated our anti-MARK4L antibody. We demonstrated that the endogenous MARK4L: (i) colocalizes with centrosomes at all mitotic stages and resides in centrosome-enriched fractions; (ii) associates with the nucleolus and the midbody and respective fractions, and (iii) co-stains the aberrant centrosome configurations observed in glioma cell lines. Conclusion: The overall data merge on the multiplex entry of MARK4L into the cell cycle and link it to the aberrant centrosomes in glioma cell lines suggesting a possible role of this kinase in the abnormal mitotic processes of human glioma.
Multiple localization of endogenous MARK4L protein in human glioma / I. Magnani, C. Novielli, M. Bellini, G. Roversi, L. Bello, L. Larizza. - In: CELLULAR ONCOLOGY. - ISSN 1570-5870. - 31:5(2009), pp. 357-370.
|Titolo:||Multiple localization of endogenous MARK4L protein in human glioma|
MAGNANI, IVANA (Primo)
NOVIELLI, CHIARA (Secondo)
BELLO, LORENZO (Penultimo)
LARIZZA, LIDIA (Ultimo)
|Parole Chiave:||Centrosome abnormalities; Glioma; Immunodetection; MARK4L; Multiple subcellular localization|
|Settore Scientifico Disciplinare:||Settore MED/27 - Neurochirurgia|
Settore MED/03 - Genetica Medica
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3233/CLO-2009-0481|
|Appare nelle tipologie:||01 - Articolo su periodico|