Among the abiotic stresses exacerbated by climate change, soil salinity is one of the most harmful for crops. Rice (Oryza sativa L.) is a very salt-sensitive cereal, with particular regard to the cultivars belonging to the subspecies japonica. In order to identify genomic traits conferring salt tolerance, a collection of 277 rice accessions (ssp. japonica) has been phenotypized under mild-salinity considering germination kinetic parameters and seedling emergence rate. Genotyping By Sequencing (GBS) followed by a Genome Wide Association Study (GWASs) were carried out. A total of 31.421 SNPs were used for the analysis. Significant genotypic-phenotypic associations were observed and a few Marker-Trait Associations (MTAs) were identified. By alignment with the rice reference genome (Os-Nipponbare-Reference-IRGSP-1.0), some of the genes putatively involved in salt stress were highlighted. Among them, our interest has been focused on OsTPP7 (chromosome 9) and OsTPP10 (chromosome 7), genes that belong to the OsTPP gene family encoding for Trehalose-6-Phosphate Phosphatase (TPP) enzymes catalyzing the dephosphorylation of Trehalose-6-Phosphate (T6P) to Trehalose (Tre). The level of T6P plays a central role in abiotic stress tolerance, acting as a signal in the cascade of events regulating sugar metabolism (sucrose) during seed germination and seedling growth. This activity is mediated by Snf1-Related protein Kinase-1 (SnRK1), a metabolic sensor fundamental in maintaining carbon homeostasis under stress condition. Biochemical and molecular analyses were performed on two rice accessions showing opposite behavior under salt stress (Olcenengo, tolerant, and SR113, sensitive). Results concerning T6P, Tre and sucrose levels in growing embryos, the evaluation of the time course of α-amylase enzyme (target of the SnRK1 system) activity and of OsTPP10-OsTPP7 gene expression appear to allow us to define a picture coherent with the different effects of salt stress in Olcenengo and SR113. Functional characterization of OsTPP10 gene and its allele mining analysis within the 277 rice accessions are in progress.

A possible role of the trehalose/trehalose-6-phosphate/SnRK1 system in rice response to salt stress / F. Colombo, V. Cavallaro, N. Negrini, S. Morgutti, A. Tondelli, G. Orasen, F.F. Nocito, G.A. Sacchi. ((Intervento presentato al 63. convegno SIGA tenutosi a Napoli nel 2019.

A possible role of the trehalose/trehalose-6-phosphate/SnRK1 system in rice response to salt stress

F. Colombo
;
V. Cavallaro;N. Negrini;S. Morgutti;G. Orasen;F.F. Nocito;G.A. Sacchi
2019

Abstract

Among the abiotic stresses exacerbated by climate change, soil salinity is one of the most harmful for crops. Rice (Oryza sativa L.) is a very salt-sensitive cereal, with particular regard to the cultivars belonging to the subspecies japonica. In order to identify genomic traits conferring salt tolerance, a collection of 277 rice accessions (ssp. japonica) has been phenotypized under mild-salinity considering germination kinetic parameters and seedling emergence rate. Genotyping By Sequencing (GBS) followed by a Genome Wide Association Study (GWASs) were carried out. A total of 31.421 SNPs were used for the analysis. Significant genotypic-phenotypic associations were observed and a few Marker-Trait Associations (MTAs) were identified. By alignment with the rice reference genome (Os-Nipponbare-Reference-IRGSP-1.0), some of the genes putatively involved in salt stress were highlighted. Among them, our interest has been focused on OsTPP7 (chromosome 9) and OsTPP10 (chromosome 7), genes that belong to the OsTPP gene family encoding for Trehalose-6-Phosphate Phosphatase (TPP) enzymes catalyzing the dephosphorylation of Trehalose-6-Phosphate (T6P) to Trehalose (Tre). The level of T6P plays a central role in abiotic stress tolerance, acting as a signal in the cascade of events regulating sugar metabolism (sucrose) during seed germination and seedling growth. This activity is mediated by Snf1-Related protein Kinase-1 (SnRK1), a metabolic sensor fundamental in maintaining carbon homeostasis under stress condition. Biochemical and molecular analyses were performed on two rice accessions showing opposite behavior under salt stress (Olcenengo, tolerant, and SR113, sensitive). Results concerning T6P, Tre and sucrose levels in growing embryos, the evaluation of the time course of α-amylase enzyme (target of the SnRK1 system) activity and of OsTPP10-OsTPP7 gene expression appear to allow us to define a picture coherent with the different effects of salt stress in Olcenengo and SR113. Functional characterization of OsTPP10 gene and its allele mining analysis within the 277 rice accessions are in progress.
set-2019
Oryza sativa ssp. japonica; salt stress; trehalose-6-P; signaling; OsTPPs
Settore AGR/13 - Chimica Agraria
Settore BIO/04 - Fisiologia Vegetale
A possible role of the trehalose/trehalose-6-phosphate/SnRK1 system in rice response to salt stress / F. Colombo, V. Cavallaro, N. Negrini, S. Morgutti, A. Tondelli, G. Orasen, F.F. Nocito, G.A. Sacchi. ((Intervento presentato al 63. convegno SIGA tenutosi a Napoli nel 2019.
Conference Object
File in questo prodotto:
File Dimensione Formato  
LXIII SIGA ANNUAL CONGRESS_Colombo et al..pdf

accesso aperto

Descrizione: Abstract
Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 83.54 kB
Formato Adobe PDF
83.54 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/682608
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact