Endothelial dysfunction is one of the primary factors in the onset and progression of atherothrombosis resulting in acute myocardial infarction (AMI). However, the pathological and cellular mechanisms of endothelial dysfunction in AMI have not been systematically studied. Protein expression profiling in combination with a protein network analysis was used by the mass spectrometry-based label-free quantification approach. This identified and quantified 2246 proteins, of which 335 were differentially regulated in coronary arterial endothelial cells from patients with AMI compared with controls. The differentially regulated protein profiles reveal the alteration of (1) metabolism of RNA, (2) platelet activation, signaling, and aggregation, (3) neutrophil degranulation, (4) metabolism of amino acids and derivatives, (5) cellular responses to stress, and (6) response to elevated platelet cytosolic Ca2+ pathways. Increased production of oxidants and decreased production of antioxidant biomarkers as well as downregulation of proteins with antioxidant properties suggests a role for oxidative stress in mediating endothelial dysfunction during AMI. In conclusion, this is the first quantitative proteomics study to evaluate the cellular mechanisms of endothelial dysfunction in patients with AMI. A better understanding of the endothelial proteome and pathophysiology of AMI may lead to the identification of new drug targets.
Differentially Expressed Proteins in Primary Endothelial Cells Derived From Patients With Acute Myocardial Infarction / S.B. Nukala, L. Regazzoni, G. Aldini, E. ZODDA, O. Tura-Ceide, N.L. Mills, M. Cascante, M. Carini, A. D'Amato. - In: HYPERTENSION. - ISSN 0194-911X. - 74:4(2019 Oct), pp. 947-956.
|Titolo:||Differentially Expressed Proteins in Primary Endothelial Cells Derived From Patients With Acute Myocardial Infarction|
NUKALA, SARATH BABU (Primo) (Corresponding)
REGAZZONI, LUCA GIOVANNI (Secondo)
CARINI, MARINA (Penultimo)
D'AMATO, ALFONSINA (Ultimo) (Corresponding)
|Parole Chiave:||coronary artery disease; mass spectrometry; proteins|
|Settore Scientifico Disciplinare:||Settore CHIM/08 - Chimica Farmaceutica|
|Progetto:||Modulation of glycolytic flux as a new approach for treatment of atherosclerosis and plaque stabilization: a multidisciplinary study (MOGLYNET)|
|Data di pubblicazione:||ott-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1161/HYPERTENSIONAHA.119.13472|
|Appare nelle tipologie:||01 - Articolo su periodico|
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