We describe two multi drug-resistant (MDR) carbapenemase-producing Escherichia coli clinical isolates from an acute hospital in Milan. Both strains, isolated from a surgical wound sample and a surveillance rectal swab respectively, were positive for a blaNDM-type gene by Xpert Carba-R test. The whole-genome sequence characterization disclosed several resistance determinants: blaNDM-5, blaCMY-42, blaTEM-198, rmtB, mphA. The two isolates belonged to phylogenetic group A, sequence type (ST) 1702 and serotype O89:H9. PCR-based replicon typing and conjugation assay demonstrated an IncI1 plasmid localization for both blaNDM-5 and blaCMY-42 genes. This is the first report of a ST1702 NDM-5 and CMY-42- producing E. coli clone in Italy.
Detection of ST1702 Escherichia coli blaNDM-5 and blaCMY-42 genes positive isolates from a Northern Italian hospital / A. Piazza, F. Comandatore, F. Romeri, C. Pagani, V. Mattioni Marchetti, M. Brilli, S. Panelli, R. Migliavacca, A. Ridolfo, P. Olivieri, M.R. Gismondo, C. Bandi, S.G. Rimoldi. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - 41:3(2018 Jul), pp. 230-231.
Titolo: | Detection of ST1702 Escherichia coli blaNDM-5 and blaCMY-42 genes positive isolates from a Northern Italian hospital |
Autori: | RIMOLDI, SARA GIORDANA (Corresponding) |
Parole Chiave: | CMY-42; Escherichia coli; NDM-5; ST1702; Aged; Anti-Bacterial Agents; Bacterial Proteins; Escherichia coli; Escherichia coli Infections; Female; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Hospitals; Humans; Italy; Male; Microbial Sensitivity Tests; Middle Aged; Phylogeny; Rectum; Surgical Wound Infection; beta-Lactamases |
Settore Scientifico Disciplinare: | Settore MED/07 - Microbiologia e Microbiologia Clinica Settore BIO/19 - Microbiologia Generale |
Data di pubblicazione: | lug-2018 |
Rivista: | |
URL: | http://www.newmicrobiologica.org/en/Previous_Issues/2018/July_2018/ |
Tipologia: | Article (author) |
Appare nelle tipologie: | 01 - Articolo su periodico |
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