Hydrocarbon (HC)pollution is a threat to the marine environment and bioremediation strategies based on microbial degradation have been developed for pollution clean-up. Effectiveness of bioaugmentation, i.e. the addition of suitable HC-degrading microorganisms to the polluted matrix, strongly depends on the metabolic and physiological versatility of cultivable HC-degrading microorganisms and on their adaptation capacity. The aim of this work was to investigate the potential of laboratory enrichment approaches to obtain cultivable HC-degrading bacteria having versatility breadth. Despite we used as inoculum marine samples of different origin and contamination history, and applied different enrichment strategies, we brought into culture 183 hydrocarbonoclastic bacterial strains strongly dominated by only the two genera Alcanivorax and Marinobacter. These isolates, screened for traits related to HC degradation, biostimulation and abiotic stress tolerance, demonstrated nevertheless to have a diverse functional potential, correlated to the adopted enrichment strategy. Although the obtained strains resulted phylogenetically similar, we showed that multiple cultivation approaches enhanced their metabolic diversification with potential benefits for bioaugmentation effectiveness.
Cultivable hydrocarbon degrading bacteria have low phylogenetic diversity but highly versatile functional potential / M. Barbato, F. Mapelli, E. Crotti, D. Daffonchio, S. Borin. - In: INTERNATIONAL BIODETERIORATION & BIODEGRADATION. - ISSN 0964-8305. - 142(2019), pp. 43-51.
|Titolo:||Cultivable hydrocarbon degrading bacteria have low phylogenetic diversity but highly versatile functional potential|
BARBATO, MARTA (Primo)
BORIN, SARA (Corresponding)
|Parole Chiave:||Alcanivorax; Bacterial diversity; Bioaugmentation; Cultivation strategies; Marine bioremediation; Marinobacter|
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
|Progetto:||Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea|
Integrated Biotechnological Solutions for Combating Marine Oil Spills
|Data di pubblicazione:||2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.ibiod.2019.04.012|
|Appare nelle tipologie:||01 - Articolo su periodico|