To increase the uptake of biocatalytic processes by industry, it is essential to demonstrate the reliability of enzyme-based methodologies directly applied to the production of high value products. Here, a unique, efficient, and sustainable enzymatic platform for the multi-gram synthesis of melatonin, projected to generate around 1.5 billion U.S. dollars worldwide by 2021, and its analogues was developed. The system exploits the covalent immobilization of MsAcT (transferase from Mycobacterium smegmatis) onto agarose beads increasing the robustness and longevity of the immobilized biocatalyst. The fully-automated process deriving from the integration between biocatalysis and flow chemistry is designed to maximize the overall yields (58-92%) and reduce reaction times (5 min), overcoming the limitation often associated with bioprocesses and bridging the gap between lab scale and industrial production.
Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process / M.L. Contente, S. Farris, L. Tamborini, F.E. Molinari, F. Paradisi. - In: GREEN CHEMISTRY. - ISSN 1463-9262. - 21(2019), pp. 3263-3266. [10.1039/c9gc01374a]
Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process
M.L. ContentePrimo
;S. FarrisSecondo
;L. Tamborini
;F.E. Molinari;
2019
Abstract
To increase the uptake of biocatalytic processes by industry, it is essential to demonstrate the reliability of enzyme-based methodologies directly applied to the production of high value products. Here, a unique, efficient, and sustainable enzymatic platform for the multi-gram synthesis of melatonin, projected to generate around 1.5 billion U.S. dollars worldwide by 2021, and its analogues was developed. The system exploits the covalent immobilization of MsAcT (transferase from Mycobacterium smegmatis) onto agarose beads increasing the robustness and longevity of the immobilized biocatalyst. The fully-automated process deriving from the integration between biocatalysis and flow chemistry is designed to maximize the overall yields (58-92%) and reduce reaction times (5 min), overcoming the limitation often associated with bioprocesses and bridging the gap between lab scale and industrial production.File | Dimensione | Formato | |
---|---|---|---|
Green_Chem_Tamborini_revised.pdf
accesso aperto
Tipologia:
Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione
428.53 kB
Formato
Adobe PDF
|
428.53 kB | Adobe PDF | Visualizza/Apri |
c9gc01374a.pdf
accesso aperto
Tipologia:
Publisher's version/PDF
Dimensione
607.71 kB
Formato
Adobe PDF
|
607.71 kB | Adobe PDF | Visualizza/Apri |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.