The transduction of Notch signal plays an intricate role in cell differentiation and pathogenesis of haematological malignancies as well as in certain congenital conditions. We found no genomic changes in either gene in 34 leukaemic samples and 25 leukaemia and lymphoma cell lines. The functionality of Notch signalling was tested using HES1 gene activation. Weshow that Notch signalling is differentially regulated in T-acute lymphoblastic leukaemia (ALL) and B-lymphoma cells. The Notch pathway is intact in a majority of B-lymphoma cell lines, but EBNA2, which mimics notch function, can occasionally activate the pathway. In contrast, the Notch pathway is constitutively active in T-ALL. This is the first demonstration of a distinction between B-lymphomas and T-cell leukaemias in the functioning of the Notch-signalling pathway. This might be related to their pathogenesis.
|Titolo:||Differential regulation of Notch signal transduction in leukemia and lymphoma cell in culture.|
|Autori interni:||CHIARAMONTE, RAFFAELLA (Primo)|
CALZAVARA, ELISABETTA (Secondo)
COMI, PAOLA PIERA MARIA
|Parole Chiave:||EBNA; HES1; Leukaemia; Lymphoma; Notch signalling|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||2003|
|Digital Object Identifier (DOI):||10.1002/jcb.10383|
|Appare nelle tipologie:||01 - Articolo su periodico|
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