Objective: To investigate the plasma levels of lopinavir by enzyme-linked immunosorbent assay (ELISA) in a cohort of patients who were vertically infected with human immunodeficiency virus 1 (HIV). Methods: Plasma levels of lopinavir (Cmin) were determined by ELISA test in patients treated with lopinavir/ritonavir-based combined antiretroviral therapy who had achieved virological response after 4 wk of therapy. Reference lopinavir concentrations were Cmin 1-8 μg/mL. Correlation between lopinavir plasma concentration and continuous variables was evaluated by mean of Pearson correlation coefficient. Differences in lopinavir (LPV) concentration for binary categorical variables were assessed by Mann-Whitney test, while for variables with more than two categories Kruskal-Wallis test was used. Results: Thirty-four patients were enroled; median age was 133 mo (15-265). The median lopinavir dose tested was 383.5 mg/kg (IQR: 266.6-400 mg/kg), with a median plasma concentration of 8.8 μg/mL (IQR: 5-14 μg/mL). Lopinavir Cmin was <1 μg/mL in only one sample (2.9 %), while 14 samples had C min between 1 and 8 μg/mL (41.2 %) and 19 (55.9 %)>8 μg/mL. No significant correlations were found between plasma concentrations of lopinavir and the continuous variables considered in the study. A negative but, not completely significant, correlation was found between plasma drug concentration and body mass index (r=-0.29; p=0.09). Conclusions: The use of a simple and relatively cost-effective methodology might render therapeutic drug monitoring (TDM) appeal in the daily clinical practice.
Pharmacokinetics of lopinavir determined with an ELISA test in youths with perinatally acquired HIV / R. Prinapori, R. Rosso, A. Di Biagio, F. Miletich, E. Furfaro, L. Taramasso, F. Ginocchio, V. Giacomet, L. Nulvesu, M.P. Sormani, I. Schiavetti, A. Signori, L. De Hoffer, C. Viscoli. - In: INDIAN JOURNAL OF PEDIATRICS. - ISSN 0019-5456. - 81:9(2014), pp. 856-860.
|Titolo:||Pharmacokinetics of lopinavir determined with an ELISA test in youths with perinatally acquired HIV|
|Parole Chiave:||Lopinavir/Ritonavir; Vertical transmission; Therapeutic drug monitoring; ELISA-TDM; HIV-infected patients; Adherence to cART|
|Settore Scientifico Disciplinare:||Settore MED/38 - Pediatria Generale e Specialistica|
|Data di pubblicazione:||2014|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s12098-013-1198-1|
|Appare nelle tipologie:||01 - Articolo su periodico|
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