Meniscus repair is still a challenge for orthopaedic surgeons as this tissue has a scarce healing potential due to the limited vascularization and to the lack of progenitor cells. Lately, several tissue engineering strategies combining cells and scaffolds have been developed. Adipose-derived stem cells (ASCs) represent a novel cell source for meniscus repair as they are easy to harvest, and they possess an intrinsic chondrogenic potential. The aim of our study was to analyze and compare the chondrogenic differentiation of swine ASCs cultured in vitro with different supplemented media. We isolated ASCs from swine adipose tissue and we placed in pellet cultures supplemented with either Bone Morphogenetic Protein (BMP)-2 and Transforming Growth Factor (TGF)-β3 or with Bone Morphogenetic Protein (BMP)-7 and Transforming Growth Factor (TGF)-β1. Samples were analyzed after 7, 14 or 21 days of culture by biochemical, histological and gene expression analysis. Our preliminary results show that BMP-2 and TGF-β3 are stronger inducers of chondrogenesis.

Characterization of different in vitro culture conditions to induce a fibro-chondrogenic differentiation of swine adipose-derived stem cells / I. Tessaro, A. Di Giancamillo, E. Benasciutti, V.T. Nguyen, U. Polito, L. Mangiavini, G.M. Peretti. - In: JOURNAL OF BIOLOGICAL REGULATORS & HOMEOSTATIC AGENTS. - ISSN 0393-974X. - 32:6 Suppl. 1(2018 Nov 01), pp. 97-103.

Characterization of different in vitro culture conditions to induce a fibro-chondrogenic differentiation of swine adipose-derived stem cells

I. Tessaro
Primo
;
A. Di Giancamillo
Secondo
;
E. Benasciutti;U. Polito;L. Mangiavini
Penultimo
;
G.M. Peretti
Ultimo
2018-11-01

Abstract

Meniscus repair is still a challenge for orthopaedic surgeons as this tissue has a scarce healing potential due to the limited vascularization and to the lack of progenitor cells. Lately, several tissue engineering strategies combining cells and scaffolds have been developed. Adipose-derived stem cells (ASCs) represent a novel cell source for meniscus repair as they are easy to harvest, and they possess an intrinsic chondrogenic potential. The aim of our study was to analyze and compare the chondrogenic differentiation of swine ASCs cultured in vitro with different supplemented media. We isolated ASCs from swine adipose tissue and we placed in pellet cultures supplemented with either Bone Morphogenetic Protein (BMP)-2 and Transforming Growth Factor (TGF)-β3 or with Bone Morphogenetic Protein (BMP)-7 and Transforming Growth Factor (TGF)-β1. Samples were analyzed after 7, 14 or 21 days of culture by biochemical, histological and gene expression analysis. Our preliminary results show that BMP-2 and TGF-β3 are stronger inducers of chondrogenesis.
Endocrinology, Diabetes and Metabolism; Immunology and Allergy; Physiology; Immunology; Oncology; Endocrinology; Physiology (medical); Cancer Research
Settore VET/01 - Anatomia degli Animali Domestici
Settore MED/33 - Malattie Apparato Locomotore
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/619759
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