The mechanisms leading to self-assembly of misfolded proteins into amyloid aggregates have been studied extensively in the test tube under well-controlled conditions. However, to what extent these processes are representative of those in the cellular environment remains unclear. Using super-resolution imaging of live cells, we show here that an amyloidogenic polyglutamine-containing protein first forms small, amorphous aggregate clusters in the cytosol, chiefly by diffusion. Dynamic interactions among these clusters limited their elongation and led to structures with a branched morphology, differing from the predominantly linear fibrils observed in vitro Some of these clusters then assembled via active transport at the microtubule-organizing center and thereby initiated the formation of perinuclear aggresomes. Although it is widely believed that aggresome formation is entirely governed by active transport along microtubules, here we demonstrate, using a combined approach of advanced imaging and mathematical modeling, that diffusion is the principal mechanism driving aggresome expansion. We found that the increasing surface area of the expanding aggresome increases the rate of accretion caused by diffusion of cytosolic aggregates and that this pathway soon dominates aggresome assembly. Our findings lead to a different view of aggresome formation than that proposed previously. We also show that aggresomes mature over time, becoming more compacted as the structure grows. The presence of large perinuclear aggregates profoundly affects the behavior and health of the cell, and our super-resolution imaging results indicate that aggresome formation and development are governed by highly dynamic processes that could be important for the design of potential therapeutic strategies.
Live-cell super-resolution microscopy reveals a primary role for diffusion in polyglutamine-driven aggresome assembly / M. Lu, L. Banetta, L.J. Young, E.J. Smith, G.P. Bates, A. Zaccone, G.S. Kaminski Schierle, A. Tunnacliffe, C.F. Kaminski. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 294:1(2019 Jan 04), pp. 257-268.
|Titolo:||Live-cell super-resolution microscopy reveals a primary role for diffusion in polyglutamine-driven aggresome assembly|
|Parole Chiave:||aggresome formation; amyloid protein; live cell SIM; molecular dynamics; molecular imaging; molecular modeling; passive transport; protein aggregation; protein misfolding; transport|
|Settore Scientifico Disciplinare:||Settore FIS/02 - Fisica Teorica, Modelli e Metodi Matematici|
Settore FIS/03 - Fisica della Materia
|Data di pubblicazione:||4-gen-2019|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1074/jbc.RA118.003500|
|Appare nelle tipologie:||01 - Articolo su periodico|