The physical nature of the bacterial chromosome has important implications for its function. Using high-resolution dynamic tracking, we observe the existence of rare but ubiquitous 'rapid movements' of chromosomal loci exhibiting near-ballistic dynamics. This suggests that these movements are either driven by an active machinery or part of stress-relaxation mechanisms. Comparison with a null physical model for subdiffusive chromosomal dynamics shows that rapid movements are excursions from a basal subdiffusive dynamics, likely due to driven and/or stress-relaxation motion. Additionally, rapid movements are in some cases coupled with known transitions of chromosomal segregation. They do not co-occur strictly with replication, their frequency varies with growth condition and chromosomal coordinate, and they show a preference for longitudinal motion. These findings support an emerging picture of the bacterial chromosome as off-equilibrium active matter and help developing a correct physical model of its in vivo dynamic structure.
Persistent super-diffusive motion of Escherichia coli chromosomal loci / A. Javer, N.J. Kuwada, Z. Long, V.G. Benza, K.D. Dorfman, P.A. Wiggins, P. Cicuta, M. Cosentino Lagomarsino. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 5(2014 May 29).
|Titolo:||Persistent super-diffusive motion of Escherichia coli chromosomal loci|
COSENTINO LAGOMARSINO, MARCO (Ultimo) (Corresponding)
|Parole Chiave:||Chromosome Segregation; Chromosomes, Bacterial; DNA Replication; Escherichia coli; Genetic Loci; Motion|
|Settore Scientifico Disciplinare:||Settore FIS/02 - Fisica Teorica, Modelli e Metodi Matematici|
Settore BIO/11 - Biologia Molecolare
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore FIS/03 - Fisica della Materia
|Data di pubblicazione:||29-mag-2014|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/ncomms4854|
|Appare nelle tipologie:||01 - Articolo su periodico|