Improperly attached chromosomes activate the mitotic checkpoint that arrests cell division before anaphase. Cells can maintain an arrest for several hours but eventually will resume proliferation, a process we refer to as adaptation. Whether adapting cells bypass an active block or whether the block has to be removed to resume proliferation is not clear. Likewise, it is not known whether all cells of a genetically homogeneous population are equally capable to adapt. Here, we show that the mitotic checkpoint is operational when yeast cells adapt and that each cell has the same propensity to adapt. Our results are consistent with a model of the mitotic checkpoint where adaptation is driven by random fluctuations of APC/C , the molecular species inhibited by the checkpoint. Our data provide a quantitative framework for understanding how cells overcome a constant stimulus that halts cell cycle progression.

Cells Escape an Operational Mitotic Checkpoint through a Stochastic Process / P. Bonaiuti, E. Chiroli, F. Gross, A. Corno, C. Vernieri, M. Štefl, M. Cosentino Lagomarsino, M. Knop, A. Ciliberto. - In: CURRENT BIOLOGY. - ISSN 1879-0445. - 28:1(2018), pp. 28-37. [10.1016/j.cub.2017.11.031]

Cells Escape an Operational Mitotic Checkpoint through a Stochastic Process

P. Bonaiuti;F. Gross;A. Corno;C. Vernieri;M. Cosentino Lagomarsino;
2018

Abstract

Improperly attached chromosomes activate the mitotic checkpoint that arrests cell division before anaphase. Cells can maintain an arrest for several hours but eventually will resume proliferation, a process we refer to as adaptation. Whether adapting cells bypass an active block or whether the block has to be removed to resume proliferation is not clear. Likewise, it is not known whether all cells of a genetically homogeneous population are equally capable to adapt. Here, we show that the mitotic checkpoint is operational when yeast cells adapt and that each cell has the same propensity to adapt. Our results are consistent with a model of the mitotic checkpoint where adaptation is driven by random fluctuations of APC/C , the molecular species inhibited by the checkpoint. Our data provide a quantitative framework for understanding how cells overcome a constant stimulus that halts cell cycle progression.
adaptation; mathematical models; mitotic checkpoint; molecular network dynamics; spindle assembly checkpoint
Settore BIO/11 - Biologia Molecolare
Settore FIS/02 - Fisica Teorica, Modelli e Metodi Matematici
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
2018
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/608072
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 10
social impact