Leaf beetles (Coleoptera: Chrysomelidae), with more than 37,000 species worldwide and about 2,300 in the Euro-Mediterranean region, are an ecological and economical relevant family, making their molecular identification of interest also in agriculture. This study, part of the Mediterranean Chrysomelidae Barcoding project (www.c-bar.org), aims to: (i) develop a reference Cytochrome c oxidase I (COI) library for the molecular identification of the Euro-Mediterranean Chrysomelidae; (ii) test the efficiency of DNA barcoding for leaf beetles identification; (iii) develop and compare optimal thresholds for distance-based identifications estimated at family and subfamily level, minimizing false positives and false negatives. Within this study, 889 COI nucleotide sequences of 261 species were provided; after the inclusion of information from other sources, a dataset of 7,237 sequences (542 species) was analysed. The average intra-interspecific distances were in the range of those recorded for Coleoptera: 1.6–24%. The estimated barcoding efficiency (~94%) confirmed the usefulness of this tool for Chrysomelidae identification. The few cases of failure were recorded for closely related species (e.g., Cryptocephalus marginellus superspecies, Cryptocephalus violaceus - Cryptocephalus duplicatus and some Altica species), even with morphologically different species sharing the same COI haplotype. Different optimal thresholds were achieved for the tested taxonomic levels, confirming that group-specific thresholds significantly improve molecular identifications.
Barcoding of Chrysomelidae of Euro-Mediterranean area : efficiency and problematic species / G. Magoga, D.C. Sahin, D. Fontaneto, M. Montagna. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018 Sep), pp. 13398.1-13398.9.
|Titolo:||Barcoding of Chrysomelidae of Euro-Mediterranean area : efficiency and problematic species|
MAGOGA, GIULIA (Primo)
MONTAGNA, MATTEO (Ultimo) (Corresponding)
|Settore Scientifico Disciplinare:||Settore AGR/11 - Entomologia Generale e Applicata|
Settore BIO/05 - Zoologia
|Data di pubblicazione:||set-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41598-018-31545-9|
|Appare nelle tipologie:||01 - Articolo su periodico|