Background and aim. Cytoskeleton and tight junctions play a pivotal role in maintaining the integrity of the intestinal barrier. A damage of these structures is involved in the pathogenesis of celiac disease. Our aim is to investigate the direct effect of gliadin on the tight junction proteins in an in vitro three-dimensional model. Materials and methods. Lovo multicellular spheroids were treated with digested bread wheat gliadin 500 microg/mL. At the end of the treatment morphology and structure of the spheroids were evaluated by means of light microscopy, scanning and transmission electron microscopy. The effect of gliadin on actin and the tight-junction proteins, occludin and zonula occludens-1, was evaluated by means of confocal laser microscopy. Results. The gliadin-treated spheroids developed holes and superficial blebs and showed profound ultrastrucural rearrangement and tight junction alterations. Incubation of Lovo spheroids with gliadin reduced the intracellular actin filaments and disassembled the integrity of the tight junction proteins. Conclusion. Our data obtained from a three-dimensional cell culture model demonstrate the direct noxious effect of gliadin on the cytoskeleton and tight junctions of Lovo cell line and suggest a role in the pathogenesis of celiac disease.
|Titolo:||Gliadin can Alter Tight Junctions Proteins in an In Vitro Model|
|Data di pubblicazione:||2-feb-2008|
|Settore Scientifico Disciplinare:||Settore MED/12 - Gastroenterologia|
|Citazione:||Gliadin can Alter Tight Junctions Proteins in an In Vitro Model ; relatore: D. Conte ; correlatore: M.T. Bardella. - Milano : Università degli studi di Milano. DIPARTIMENTO DI SCIENZE MEDICHE, 2008 Feb 02. ((19. ciclo, Anno Accademico 2006/2007.|
|Digital Object Identifier (DOI):||10.13130/elli-luca_phd2008-02-02|
|Appare nelle tipologie:||13 - Tesi di dottorato discussa entro ottobre 2010|