IRIS Institutional Research Information System - AIR Archivio Istituzionale della Ricerca

IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Milano.

AIR nasce in UNIMI nel 2006 con lo scopo di raccogliere, documentare e conservare le informazioni sulla produzione scientifica dell'Università degli Studi di Milano e costituisce di fatto l'Anagrafe della ricerca dell'Ateneo.

In veterinary medicine, assay performance is often affected by the lack of species-specific diagnostic tools. Reliable biomarkers might be identified by investigating biological fluids of the species of interest, but protein sequence databases are often incomplete and human-specific devices for reducing sample complexity might fail when applied to animal plasma. Here, seven commercial methods based on different capturing agents (anti-human antibodies, affinity ligands, mixture of antibodies and ligands, and combinatorial peptide ligand libraries) were applied to cat plasma and evaluated in terms of yield, identified proteins/ peptides, and relative abundance by high resolution shotgun proteomics and label-free quantitation. As a result, anti-human antibody-based methods were unsatisfactory. Most failed in reducing albumin and immunoglobulins, and some led to a substantial removal of other highly abundant proteins, probably because of nonspecific interactions. A protein A/dye ligand-based method was efficient in reducing immunoglobulins, fibrinogen and apolipoprotein A1 and A2, but not albumin, and protein identifications did not increase. Only peptide ligand libraries flattened the dynamic range, and increased protein identification (59.0%). Albumin and immunoglobulins were successfully depleted (60.7% and 35.9%, respectively). Although further studies will be required for reinforcing our observations, this work can provide a useful guide for cat plasma pretreatment in biomarker discovery studies.

All Cats Are Grey in the Dark: Enrichment/Depletion Approaches for Biomarker Discovery on Felis catus Plasma / L. Carcangiu, S. Pisanu, S. Tore, M.F. Addis, E. Zini, S. Uzzau, D. Pagnozzi. - In: PROTEOMICS. - ISSN 1615-9853. - 18:20(2018 Oct).

Powered by IRIS-about IRIS-Utilizzo dei cookie

 Copyright © 2020