Galactose, a marker of heat treatment, has been analysed in milk as pentafluorobenzyloxime acetate by gas chromatography with flame ionization detection using a simple switching valve system. The procedure did not entail any pre-derivatization clean-up for lactose elimination from the sample. In a short pre-column, reagent and lactose derivative excess were separated and the galactose and internal standard derivatives were transferred to the analytical column by a four-port valve. Thus, the analytical column was protected from overloading, so avoiding rapid deterioration.

Gas chromatographic determination of galactose in milk : example of a switching valve used for the protection of the capillary column / L.M. Chiesa, L. Radice, R. Belloli, P. Renon, P.A. Biondi. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 847:1/2(1999 Jun 25), pp. 47-51.

Gas chromatographic determination of galactose in milk : example of a switching valve used for the protection of the capillary column

L.M. Chiesa
Primo
;
P. Renon
Penultimo
;
P.A. Biondi
Ultimo
1999-06-25

Abstract

Galactose, a marker of heat treatment, has been analysed in milk as pentafluorobenzyloxime acetate by gas chromatography with flame ionization detection using a simple switching valve system. The procedure did not entail any pre-derivatization clean-up for lactose elimination from the sample. In a short pre-column, reagent and lactose derivative excess were separated and the galactose and internal standard derivatives were transferred to the analytical column by a four-port valve. Thus, the analytical column was protected from overloading, so avoiding rapid deterioration.
Derivatization, GC; Food analysis; Galactose; Milk; Pentafluorobenzyloxime acetate
Settore VET/04 - Ispezione degli Alimenti di Origine Animale
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/58361
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