Background: Interleukin-21 (IL-21) modulates HIV-1 infection through the elicitation of different antiviral mechanisms, including Th17 lineage commitment and induction of microRNA (miR)-29,a mi RNA endowed with anti-HIV activity. As miR-29 expression is significantly increased in HIV-1-exposed seronegative individuals (HESN), we investigated the role of miR-29/IL21 axis in the natural control of HIV-1 infection. Methods: Peripheral blood mononuclear cells (PBMCs) isolated from 15 Italian sexually exposed HESN and 15 HIV-unexposed healthy controls were in-vitro infected with an R5-tropic HIV-1(Ba-L) strain. Seven days post HIV-1 infection we evaluated: 1) p24 production (ELISA); 2) CD4(+)/IL-21(+) and CD4(+)/IL-17(+) T lymphocytes (FACS); 3) IL-17 concentration in supernatants (ELISA); and 4) IL-6, IL-17, IL-21, and miR-29a,b,c expression by CD4(+) T lymphocytes as well as perforin and granzyme by peripheral blood mononuclear cells (qPCR). The same analyses were performed on the 15 HIV-positive partners. Results: At baseline IL-6 expression alone was increased in HESN compared to healthy controls. Seven days after in-vitro HIV-1 infection, nevertheless, differences emerged. Thus, CD4(+)/IL21(+) and CD4(+)/IL17(+) T lymphocytes, as well as IL-21 and IL-17 expression and production were significantly augmented in HESN compared to healthy controls. Interestingly, IL-21 upregulation correlated with a significantly increased expression of miR-29a,b,c and a reduced susceptibility to in-vitro HIV-1 infection in HESN alone. No differences were observed in perforin and granzyme expression. Conclusion: The IL-21/miR-29 axis is upregulated by HIV-1 infection in HESN suggesting its involvement in the natural resistance to HIV-1 infection in HESN. Approaches that exogenously increase IL-21 production or prompt preexisting cellular IL-21 reservoir could confine the magnitude of the initial HIV-1 infection.
The interleukin 21 (IL 21)/ microRNA-29 (miR-29) axis is associated with natural resistance to HIV-1 infection / P.A.S. Ortega, I. Saulle, V. Mercurio, S.V. Ibba, E.M. Lori, C. Fenizia, M. Masetti, D. Trabattoni, S.L. Caputo, F. Vichi, F. Mazzotta, M. Clerici, M. Biasin. - In: AIDS. - ISSN 0269-9370. - 32:17(2018 Nov 13), pp. 2453-2461.
|Titolo:||The interleukin 21 (IL 21)/ microRNA-29 (miR-29) axis is associated with natural resistance to HIV-1 infection|
SERNA ORTEGA, PAULA ANDREA (Primo)
SAULLE, IRMA (Secondo)
CLERICI, MARIO SALVATORE (Penultimo)
BIASIN, MARA (Ultimo) (Corresponding)
|Parole Chiave:||HIV-1-exposed seronegative individual; HIV; interleukin-21; miRNA-29|
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
Settore MED/04 - Patologia Generale
|Data di pubblicazione:||13-nov-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1097/QAD.0000000000001938|
|Appare nelle tipologie:||01 - Articolo su periodico|