Fragile X mental retardation-related protein 1 (FXR1) is a cytoplasmic RNA-binding protein highly conserved among vertebrates. It has been studied for its role in muscle development, inflammation, and tumorigenesis, being related, for example, to metastasizing behavior in human and canine uveal melanoma. Anti-FXR1 antibodies have never been validated in the canine species. To investigate FXR1 expression in canine melanocytic tumors, the present study tested two commercially available polyclonal anti-human FXR1 antibodies, raised in goat and rabbit, respectively. The cross-reactivity of the anti-FXR1 antibodies was assessed by Western blot analysis, and the protein was localized by IHC in a set of normal canine tissues and in canine melanocytic tumors (10 uveal and 10 oral). Western blot results demonstrated that the antibody raised in rabbit specifically recognized the canine FXR1, while the antibody raised in goat did not cross-react with this canine protein. FXR1 protein was immunodetected using rabbit anti-FXR1 antibody, in canine normal tissues with different levels of intensity and distribution. It was also detected in 10/10 uveal and 9/10 oral melanocytic tumors. The present study validated for the first time the use of anti-FXR1 antibody in dogs and highlighted different FXR1 protein expression in canine melanocytic tumors, the significance of which is undergoing further investigations.
Immunohistochemical Expression of FXR1 in Canine Normal Tissues and Melanomas / L. Nordio, A.T. Marques, C. Lecchi, A.M. Luciano, D. Stefanello, C. Giudice. - In: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. - ISSN 0022-1554. - 66:8(2018 Aug), pp. 585-593.
|Titolo:||Immunohistochemical Expression of FXR1 in Canine Normal Tissues and Melanomas|
NORDIO, LAURA (Primo) (Corresponding)
STEFANELLO, DAMIANO (Penultimo)
GIUDICE, CHIARA (Ultimo)
|Parole Chiave:||dog, eye, FXR1, IHC, melanoma, neoplasia, oral cavity, Western blot|
|Settore Scientifico Disciplinare:||Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria|
|Data di pubblicazione:||ago-2018|
|Data ahead of print / Data di stampa:||2-apr-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1369/0022155418766292|
|Appare nelle tipologie:||01 - Articolo su periodico|