The presence of psychrophilic yeasts in supra- and subglacial sediments, ice and meltwater collected from two glaciers of the Italian Alps (Forni and Sforzellina – Ortles-Cevedale group) was investigated. After incubation at 4°C, subglacial sediments contained from 1.3 x 10^3 to 9.6 x 10^3 CFU of yeasts g^(-1). The number of yeast cells in supraglacial sediments was c. 10–100-fold lower. A significant proportion of isolated yeasts exhibited one or more extracellular enzymatic activities (starch-degrading, lipolytic, esterolytic, proteolytic and pectinolytic activity) at 4°C. Selected isolates were able to grow at 2°C under laboratorysimulated in situ conditions. In all, 106 isolated yeasts were identified by MSP-PCR fingerprinting and 26S rRNA gene sequencing of the D1/D2 region as belonging to 10 species: Aureobasidium pullulans, Cryptococcus gilvescens (over 50% of the total), Cryptococcus terricolus, Mrakia gelida, Naganishia globosa, Rhodotorula glacialis, Rhodotorula psychrophenolica, Rhodotorula bacarum, Rhodotorula creatinivora and Rhodotorula laryngis. Four strains, all belonging to a new yeast species, yet to be described, were also isolated.
|Titolo:||Psychrophilic yeasts in glacial environments of Alpine glaciers|
SMIRAGLIA, CLAUDIO (Penultimo)
|Parole Chiave:||Alpine glaciers; Glacial environments; Psychrophilic yeasts|
|Settore Scientifico Disciplinare:||Settore GEO/04 - Geografia Fisica e Geomorfologia|
|Data di pubblicazione:||gen-2008|
|Digital Object Identifier (DOI):||10.1111/j.1574-6941.2007.00409.x|
|Appare nelle tipologie:||01 - Articolo su periodico|