The isolation and characterization from the sand fly Phlebotomus perniciosus of a Wickerhamomyces anomalus yeast strain (Wa1F1) displaying the killer phenotype was recently reported. In the present work, the killer toxin (KT) produced by Wa1F1 was purified and characterized, and its antimicrobial activity in vitro was investigated against fluconazole- susceptible and -resistant clinical isolates and laboratory strains of Candida albicans and C. glabrata displaying known mutations. Wa1F1-KT showed a differential killing ability against different mutant strains of the same species. The results may be useful for the design of therapeutic molecules based on Wa1F1-KT and the study of yeast resistance mechanisms.
Candidacidal Activity of a Novel Killer Toxin from Wickerhamomyces anomalus against Fluconazole-Susceptible and -Resistant Strains. / L. Giovati, C. Santinoli, E. Ferrari, T. Ciociola, E. Martin, C. Bandi, I. Ricci, S. Epis, S. Conti. - In: TOXINS. - ISSN 2072-6651. - 10:2(2018 Feb 03), pp. 68.1-68.11.
|Titolo:||Candidacidal Activity of a Novel Killer Toxin from Wickerhamomyces anomalus against Fluconazole-Susceptible and -Resistant Strains.|
|Parole Chiave:||A novel killer toxin produced by the recently isolated Wickerhamomyces anomalus strain 1F1 showed a differential killing ability against fluconazole-susceptible and -resistant Candida clinical isolates and laboratory strains displaying known mutations.; Candida albicans; Candida glabrata; Key Contribution; Wickerhamomyces anomalus; antifungal resistance; yeast killer toxin|
|Settore Scientifico Disciplinare:||Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali|
|Data di pubblicazione:||3-feb-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/toxins10020068|
|Appare nelle tipologie:||01 - Articolo su periodico|