Purpose: Increasing evidence suggests the potential use of natural antioxidant compounds in the prevention/treatment of osteoporosis. This study was undertaken to investigate the effects of purified delphinidin-3-rutinoside (D3R), isolated from Solanum melongena L., on osteoblast viability and differentiation in basal conditions and its ability to protect MC3T3-E1 cells against oxidative damage induced by tert-butyl hydroperoxide (t-BHP). Methods: MC3T3-E1 osteoblastic cells were treated with D3R (10−11–10−5 M for 24 h), followed by treatment with t-BHP (250 µM for 3 h). To test cell viability, MTT test was performed. Apoptotic cells were stained with Hoechst-33258 dye. Cytoskeleton rearrangement was stained with FICT-labelled phalloidin. Intracellular ROS production was measured using dichlorofluorescein CM-DCFA. The reduced glutathione to oxidized glutathione ratio (GSH/GSSG) contents was measured according to the OPT fluorimetric assay. Results: D3R (10−9 M) significantly increases viability of MC3T3-E1 cells and promotes osteoblast differentiation by increasing the expression of type I collagen, alkaline phosphatase and osteocalcin. Pre-treatment with D3R (10−9 M) significantly prevented t-BHP-induced osteoblastic dysfunction and changes in the cytoskeleton organization by decreasing intracellular ROS and preventing the reduction in GSH/GSSG. D3R did not significantly modify the expression of Osteoprotegerin/RANKL system activated by t-BHP suggesting a lack of effect of D3R on osteoblast/osteoclast crosstalk. D3R protective effects against t-BHP-induced osteoblastic dysfunction were mediated by the PI3K/Akt pathway since they were completely prevented by LY294002, a PI3K/Akt specific inhibitor. Conclusions: These findings indicate that D3R protects MC3T3-E1 cells from oxidative damage and suggest the potential utility of dietary D3R supplement to prevent osteoblast dysfunction in age-related osteoporosis.
Potential of delphinidin-3-rutinoside extracted from Solanum melongena L. as promoter of osteoblastic MC3T3-E1 function and antagonist of oxidative damage / L. Casati, F. Pagani, M. Fibiani, R. Lo Scalzo, V. Sibilia. - In: EUROPEAN JOURNAL OF NUTRITION. - ISSN 1436-6207. - (2018 Jan 25). [Epub ahead of print]
Titolo: | Potential of delphinidin-3-rutinoside extracted from Solanum melongena L. as promoter of osteoblastic MC3T3-E1 function and antagonist of oxidative damage |
Autori: | SIBILIA, VALERIA (Corresponding) |
Parole Chiave: | delphinidin-3-rutinoside; functional food component; osteoblastic MC3T3-E1 cells; oxidative stress |
Settore Scientifico Disciplinare: | Settore BIO/14 - Farmacologia Settore BIO/13 - Biologia Applicata Settore MED/04 - Patologia Generale |
Data di pubblicazione: | 25-gen-2018 |
Rivista: | |
Tipologia: | Article (author) |
Data ahead of print / Data di stampa: | 25-gen-2018 |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/s00394-018-1618-0 |
Appare nelle tipologie: | 01 - Articolo su periodico |
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