Candida albicans and Candida dubliniensis are related yeasts that differ in the expression of virulence-associated proteins involved in adherence and biofilm development. CR3-RP (complement receptor 3-related protein) is one of the surface antigens expressed by Candida species. The main objective of this research was to elucidate the effect of the polyclonal anti-CR3-RP antibody (Ab) on adherence and the biofilm formed by C. albicans SC5314 and C. dubliniensis CBS 7987 and 2 clinical isolates in vitro, ex vivo, and in vivo. A comparison of species, and of treated vs. non-treated with the anti-CR3-RP Ab showed a reduction in adherence (22 - 41%) that was dependent on the time point of evaluation (60, 90 or 120 min), but did not prove to be species-dependent. Confocal microscopy revealed a decreased thickness in biofilms formed by both species after pre-treatment with the anti-CR3-RP Ab. This observation was confirmed ex vivo by immunohistochemistry analysis of biofilms formed on mouse tongues. Moreover anti-CR3-RP Ab administration, 1h post infection, has been shown to promote larval survival compared to the control group in a Galleria mellonella infection model.Our data suggest a potential activity of the anti-CR3-RP Ab relevant to immunotherapy or vaccine development against biofilm-associated Candida infections.
Anti-biofilm activity of antibody directed against surface antigen CR3-RP - comparison of Candida albicans and Candida dubliniensis / J. Chupácová, E. Borghi, G. Morace, A. Los, H. Bujdáková. - In: PATHOGENS AND DISEASE. - ISSN 2049-632X. - (2018 Jan 05). [Epub ahead of print] [10.1093/femspd/ftx127]
Anti-biofilm activity of antibody directed against surface antigen CR3-RP - comparison of Candida albicans and Candida dubliniensis
E. Borghi;G. MoraceSupervision
;
2018
Abstract
Candida albicans and Candida dubliniensis are related yeasts that differ in the expression of virulence-associated proteins involved in adherence and biofilm development. CR3-RP (complement receptor 3-related protein) is one of the surface antigens expressed by Candida species. The main objective of this research was to elucidate the effect of the polyclonal anti-CR3-RP antibody (Ab) on adherence and the biofilm formed by C. albicans SC5314 and C. dubliniensis CBS 7987 and 2 clinical isolates in vitro, ex vivo, and in vivo. A comparison of species, and of treated vs. non-treated with the anti-CR3-RP Ab showed a reduction in adherence (22 - 41%) that was dependent on the time point of evaluation (60, 90 or 120 min), but did not prove to be species-dependent. Confocal microscopy revealed a decreased thickness in biofilms formed by both species after pre-treatment with the anti-CR3-RP Ab. This observation was confirmed ex vivo by immunohistochemistry analysis of biofilms formed on mouse tongues. Moreover anti-CR3-RP Ab administration, 1h post infection, has been shown to promote larval survival compared to the control group in a Galleria mellonella infection model.Our data suggest a potential activity of the anti-CR3-RP Ab relevant to immunotherapy or vaccine development against biofilm-associated Candida infections.Pubblicazioni consigliate
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