Aim of this study was to set up a method by capillary electrophoresis to detect lactulose and mannitol in urine after an oral load, and to estimate the intestinal permeability in controls and in type I diabetes patients. The underivatized carbohydrates were monitored by indirect UV detection using sorbate, cetyltrimethylammonium bromide and LiOH as background electrolyte. Urines were purified by solid phase extraction, shaken with cation exchange resin, filtered and analysed. Carbohydrates migrated in <10 min in relation to their pKa and Mr. Controls (n = 33) and patients (n = 23) had an excretion ratio lactulose/mannitol 0.025 (0.018–0.051) and 0.067 (0.050–0.127), respectively (p < 0.01, median, interquartile range).

Lactulose and mannitol intestinal permeability detected by capillary electrophoresis / R. Paroni, I. Fermo, L. Molteni, L. Folini, M.R. Pastore, A. Mosca, E. Bosi. - In: JOURNAL OF CHROMATOGRAPHY. B. - ISSN 1570-0232. - 834:1-2(2006), pp. 183-187. [10.1016/j.jchromb.2006.02.050]

Lactulose and mannitol intestinal permeability detected by capillary electrophoresis

R. Paroni
Primo
;
A. Mosca
Penultimo
;
2006

Abstract

Aim of this study was to set up a method by capillary electrophoresis to detect lactulose and mannitol in urine after an oral load, and to estimate the intestinal permeability in controls and in type I diabetes patients. The underivatized carbohydrates were monitored by indirect UV detection using sorbate, cetyltrimethylammonium bromide and LiOH as background electrolyte. Urines were purified by solid phase extraction, shaken with cation exchange resin, filtered and analysed. Carbohydrates migrated in <10 min in relation to their pKa and Mr. Controls (n = 33) and patients (n = 23) had an excretion ratio lactulose/mannitol 0.025 (0.018–0.051) and 0.067 (0.050–0.127), respectively (p < 0.01, median, interquartile range).
Capillary electrophoresis; Intestinal permeability; Lactulose; Mannitol; Urine
Settore BIO/10 - Biochimica
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
2006
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/5410
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