Light chain amyloidosis (AL), the most common systemic amyloidosis, is caused by the overproduction and the aggregation of monoclonal immunoglobulin light chains (LC) in target organs. Due to genetic rearrangement and somatic hypermutation, virtually, each AL patient presents a different amyloidogenic LC. Because of such complexity, the fine molecular determinants of LC aggregation propensity and proteotoxicity are, to date, unclear; significantly, their decoding requires investigating large sets of cases. Aiming to achieve generalizable observations, we systematically characterised a pool of thirteen sequence-diverse full length LCs. Eight amyloidogenic LCs were selected as responsible for severe cardiac symptoms in patients; five non-amyloidogenic LCs were isolated from patients affected by multiple myeloma. Our comprehensive approach (consisting of spectroscopic techniques, limited proteolysis, and X-ray crystallography) shows that low fold stability and high protein dynamics correlate with amyloidogenic LCs, while hydrophobicity, structural rearrangements and nature of the LC dimeric association interface (as observed in seven crystal structures here presented) do not appear to play a significant role in defining amyloid propensity. Based on the structural and biophysical data, our results highlight shared properties driving LC amyloid propensity, and these data will be instrumental for the design of synthetic inhibitors of LC aggregation.
Concurrent structural and biophysical traits link with immunoglobulin light chains amyloid propensity / L. Oberti, P. Rognoni, A.G. Barbiroli, F. Lavatelli, R. Russo, M. Maritan, G. Palladini, M. Bolognesi, G. Merlini, S. Ricagno. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7:1(2017 Dec 01), pp. 16809.1-16809.11.
|Titolo:||Concurrent structural and biophysical traits link with immunoglobulin light chains amyloid propensity|
RICAGNO, STEFANO (Corresponding)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1-dic-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41598-017-16953-7|
|Appare nelle tipologie:||01 - Articolo su periodico|