Micro- and nanoscale objects with anisotropic shape are key components of a variety of biological systems and inert complex materials, and represent fundamental building blocks of novel self-assembly strategies. The time scale of their thermal motion is set by their translational and rotational diffusion coefficients, whose measurement may become difficult for relatively large particles with small optical contrast. Here we show that Dark Field Differential Dynamic Microscopy is the ideal tool for probing the roto-translational Brownian motion of shape anisotropic particles. We demonstrate our approach by successful application to aqueous dispersions of non-motile bacteria and of colloidal aggregates of spherical particles.

Dark field differential dynamic microscopy enables the accurate characterization of the roto-translational dynamics of bacteria and colloidal clusters / R. Cerbino, D. Piotti, M. Buscaglia, F. Giavazzi. - In: JOURNAL OF PHYSICS. CONDENSED MATTER. - ISSN 0953-8984. - 30:2(2018 Jan 17), pp. 025901.1-025901.12. [10.1088/1361-648X/aa9bc5]

Dark field differential dynamic microscopy enables the accurate characterization of the roto-translational dynamics of bacteria and colloidal clusters

R. Cerbino;M. Buscaglia;F. Giavazzi
2018-01-17

Abstract

Micro- and nanoscale objects with anisotropic shape are key components of a variety of biological systems and inert complex materials, and represent fundamental building blocks of novel self-assembly strategies. The time scale of their thermal motion is set by their translational and rotational diffusion coefficients, whose measurement may become difficult for relatively large particles with small optical contrast. Here we show that Dark Field Differential Dynamic Microscopy is the ideal tool for probing the roto-translational Brownian motion of shape anisotropic particles. We demonstrate our approach by successful application to aqueous dispersions of non-motile bacteria and of colloidal aggregates of spherical particles.
Aggregation; Bacteria; Browinan motion; Microscopy; Rotational diffusion
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore FIS/03 - Fisica della Materia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/531807
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