The SIN Caffaro is a large polluted site of national priority located in Italy, originated by the activities of a former chemical factory. The site is highly contaminated by chlorinated persistent organic pollutants, mainly constituted by polychlorobyphenils (PCBs), together with heavy metals and metalloids, in variable concentrations and uneven distribution. We aimed to explore the diversity of root-associated bacterial communities driven by different plant species spontaneously established in abandoned agricultural soils within the most polluted area in the site. The overall structure of the non-vegetated and root-associated soil fractions bacterial communities was described by high-throughput sequencing of the 16S rRNA gene, and a collection of rhizobacteria was established and tested for plant growth promotion (PGP) traits and bioremediation potential. The results showed that the recruitment of specific bacterial communities in the root-associated soil fractions was driven by both soil fractions and plant species. PCR-based detection in the soil metagenome of bacterial bphA gene, encoding for the biphenyl dioxygenase α subunit, indicated that the soil in the site possesses metabolic traits linked to PCB degradation. The cultivable microbiota in the rhizosphere of the different plant species showed highly similar phylogenetic and functional composition, in terms of PGP and bioremediation potential. 72% of the strains harbored the bphA gene and displayed catechol 2,3-dioxygenase and 46% showed ACC deaminase activities, the latter considered a PGP trait contributing to plant tolerance to environmental stress. Overall, this work highlighted that spontaneous plant species differentially affect the rhizosphere bacterial community composition and harbor the intrinsic potential to sustain natural attenuation processes, further exploitable for rhizoremediation intervention at the SIN Caffaro.
Bacteria associated to spontaneous plants in a historical PCB polluted soil show potential to sustain natural attenuation / L. Vergani, F. Mapelli, R. Marasco, E.S. Crotti, M. Fusi, D.G. Daffonchio, S. Borin. ((Intervento presentato al convegno Microbial Diversity tenutosi a Bari nel 2017.
|Titolo:||Bacteria associated to spontaneous plants in a historical PCB polluted soil show potential to sustain natural attenuation|
VERGANI, LORENZO (Primo)
MAPELLI, FRANCESCA (Secondo)
DAFFONCHIO, DANIELE GIUSEPPE (Penultimo)
BORIN, SARA (Ultimo) (Corresponding)
|Data di pubblicazione:||ott-2017|
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
|Citazione:||Bacteria associated to spontaneous plants in a historical PCB polluted soil show potential to sustain natural attenuation / L. Vergani, F. Mapelli, R. Marasco, E.S. Crotti, M. Fusi, D.G. Daffonchio, S. Borin. ((Intervento presentato al convegno Microbial Diversity tenutosi a Bari nel 2017.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|