Obesity is a pathological condition caused by genetic and environmental factors, including vitamin D deficiency, which increases the risk of developing cardiovascular disorders and diabetes. This case-control study was designed to verify whether serum profiles could be identified differentiating obese and non-obese Saudis characterized by vitamin D deficiency and pathological levels of triglycerides, high-density lipoprotein cholesterol and high total cholesterol levels. The serum protein profiles of 64 vitamin D deficient (serum 25(OH)D < 50nmol/L) individuals with metabolic syndrome and with (n = 31; BMI ≥ 30) or without (n = 33; BMI < 30) obesity were analyzed by a quantitative label-free mass spectrometry approach (MALDI-profiling), combined with different serum immunodepletion strategies (Human7 and Human14 immuno-chromatographies), to analyze the intermediate- and low-abundant protein components. The analysis of intermediate-abundant proteins (Human7) in obese vs. non-obese subjects identified 14 changed peaks (p < 0.05) in the m/z range 1500-35000. Furthermore, the Human14 depletion provided new profiles related to obesity (121 changed peaks). Among changed peaks, 11 were identified in the m/z range 1500-4000 Da by high-resolution tandem mass spectrometry, belonging to apolipoprotein CIII, apolipoprotein B100, alpha-1-antichymotrypsin and complement C3. Data herein show that distinct protein profiles identify specific peptides belonging to lipid metabolism and inflammation processes that are associated with obesity and vitamin D deficiency.
Intermediate and low abundant protein analysis of vitamin D deficient obese and non-obese subjects by MALDI-profiling / N..M. Al Daghri, E. Torretta, D. Capitanio, C. Fania, F.R. Guerini, S..B. Sabico, M.S. Clerici, C. Gelfi. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 7:1(2017 Oct 03), pp. 12633.1-12633.10.
|Titolo:||Intermediate and low abundant protein analysis of vitamin D deficient obese and non-obese subjects by MALDI-profiling|
TORRETTA, ENRICA (Secondo)
CLERICI, MARIO SALVATORE (Penultimo)
GELFI, CECILIA (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica|
|Data di pubblicazione:||3-ott-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41598-017-13020-z|
|Appare nelle tipologie:||01 - Articolo su periodico|