Structure-based epitope prediction drives the design of diagnostic peptidic probes to reveal specific antibodies elicited in response to infections. We previously identified a highly immunoreactive epitope from the peptidoglycan-associated lipoprotein (Pal) antigen from Burkholderia pseudomallei, which could also diagnose Burkholderia cepacia infections. Here, considering the high phylogenetic conservation within Burkholderia species, we ask whether cross reactivity can be reciprocally displayed by the synthetic epitope from B. cenocepacia. We perform comparative analyses of the conformational preferences and diagnostic performances of the corresponding epitopes from the two Burkholderia species when presented in the context of the full-length proteins or as isolated peptides. The effects of conformation on the diagnostic potential and cross-reactivity of Pal peptide epitopes are rationalized on the basis of the 1.8 angstrom crystal structure of B. cenocepacia Pal and through computational analyses. Our results are discussed in the context of designing new diagnostic molecules for the early detection of infectious diseases.

Designing Probes for Immunodiagnostics : Structural Insights into an Epitope Targeting Burkholderia Infections / R. Capelli, E. Matterazzo, M. Amabili, C. Peri, A. Gori, P. Gagni, M. Chiari, G. Lertmemongkolchai, M. Cretich, M. Bolognesi, G. Colombo, L.J. Gourlay. - In: ACS INFECTIOUS DISEASES. - ISSN 2373-8227. - 3:10(2017 Oct 13), pp. 736-743. [10.1021/acsinfecdis.7b00080]

Designing Probes for Immunodiagnostics : Structural Insights into an Epitope Targeting Burkholderia Infections

R. Capelli
Primo
;
E. Matterazzo
Secondo
;
C. Peri;A. Gori;P. Gagni;M. Bolognesi;L.J. Gourlay
Ultimo
2017-10-13

Abstract

Structure-based epitope prediction drives the design of diagnostic peptidic probes to reveal specific antibodies elicited in response to infections. We previously identified a highly immunoreactive epitope from the peptidoglycan-associated lipoprotein (Pal) antigen from Burkholderia pseudomallei, which could also diagnose Burkholderia cepacia infections. Here, considering the high phylogenetic conservation within Burkholderia species, we ask whether cross reactivity can be reciprocally displayed by the synthetic epitope from B. cenocepacia. We perform comparative analyses of the conformational preferences and diagnostic performances of the corresponding epitopes from the two Burkholderia species when presented in the context of the full-length proteins or as isolated peptides. The effects of conformation on the diagnostic potential and cross-reactivity of Pal peptide epitopes are rationalized on the basis of the 1.8 angstrom crystal structure of B. cenocepacia Pal and through computational analyses. Our results are discussed in the context of designing new diagnostic molecules for the early detection of infectious diseases.
Burkholderia spp.; antigen; crystal structure; epitope; immunodiagnostics; microarray
Settore BIO/10 - Biochimica
Discovery/Development of diagnostic probes and vaccine candidates targeting burkholderia infections
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/521845
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