Background: ErsA is a novel Pseudomonas aeruginosa small RNA responsive to infection-relevant host stimuli. Its transcription is under the control of the alternative sigma factor σ22 (AlgT/U), implicated in bacterial virulence. In strain PAO1, ErsA exerts a direct negative post-transcriptional regulation in an incoherent feed-forward loop with σ22 on the bi-functional enzyme AlgC, which is involved in the biosynthesis of sugar precursors for alginate and polysaccharide exoproducts. Objectives: We aim at the characterization of novel target genes regulated by ErsA to expand the knowledge about the ErsA-based regulatory network. Methods: We employed different approaches: i) bioinformatics analysis, ii) in vitro electrophoretic mobility shift assays, iii) in vivo translational fusions based on the gfp reporter gene, iv) RNA-seq, and v) biofilm and motility assays on mutant strains. Conclusions: Our results comprise: i) In silico analyses identified new putative target genes mainly involved in biofilm formation and exopolysaccharides production. ii) Among these targets, we specifically validated the interaction between ErsA and amrZ mRNA, both in vitro and in vivo iii) The RNA-seq analysis showed that some of the genes whose expression is positively controlled by ErsA are important for biofilm development (i.e. genes belonging to the pel operon, algD). iv) In line with the other results, the ErsA deletion mutant shows a hyper-motile phenotype compared to the wildtype and develops a thin and flat biofilm. Overall, our results suggest that the small RNA ErsA might represent a relevant post-transcriptional regulator in biofilm development at different levels, likely interacting with different mRNA targets.
Dissecting the role of the small RNA ErsA in Pseudomonas aeruginosa motility and biofilm regulation / M. Falcone, S. Ferrara, E. Rossi, H.K. Johansen, S. Molin, G. Bertoni. ((Intervento presentato al 7. convegno FEMS-Microbiology tenutosi a Valencia nel 2017.
|Titolo:||Dissecting the role of the small RNA ErsA in Pseudomonas aeruginosa motility and biofilm regulation|
FALCONE, MARILENA (Primo)
FERRARA, SILVIA (Secondo)
BERTONI, GIOVANNI (Ultimo)
|Data di pubblicazione:||lug-2017|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
|Citazione:||Dissecting the role of the small RNA ErsA in Pseudomonas aeruginosa motility and biofilm regulation / M. Falcone, S. Ferrara, E. Rossi, H.K. Johansen, S. Molin, G. Bertoni. ((Intervento presentato al 7. convegno FEMS-Microbiology tenutosi a Valencia nel 2017.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|