Research in our group focuses on the genetic and molecular dissection of barley development as a mean to identify useful genes for the manipulation of plant architecture. Although progress has been made in rice and maize, the molecular bases of development are largely unknown in Triticeae species. Traits under analysis include tillering, spike morphology, and development of leaves and floral bracts. In order to identify and characterize genes involved in these processes, we have been exploiting a wide collection of developmental mutants, combining candidate gene (CG) and reverse genetics approaches. CGs for inflorescence branching and foliar development were identified based on synteny between the barley and rice genomes. CGs for meristem function and floret development were identified through molecular approaches, using as a starting point the Hooded (K) mutant. Support for specific CGs was provided by phenotypic similarities between rice and barley mutants. Validation of barley CGs is based on a range of complementarity between forward and reverse genetics approaches.
Candidate gene and reverse genetics approaches for the analysis of development in barley / S. Ciannamea, M. Osnato, E. Miatton, F. Salamini, A. Druka, D. Meynard, E. Guiderdoni, C. Pozzi, L. Rossini - In: Proceedings of the 10th International Barley Genetics Symposium, 5-10 April 2008, Alexandria, Egypt / [a cura di] S. Ceccarelli, S. Grando. - Aleppo : International Center for Agricultural Research in the Dry Areas (ICARDA), 2010. - ISBN 92-9127-246-9. - pp. 142-145 (( Intervento presentato al 10. convegno Barley Genetics Symposium tenutosi a Alexandria nel 2008.
|Titolo:||Candidate gene and reverse genetics approaches for the analysis of development in barley|
|Settore Scientifico Disciplinare:||Settore AGR/07 - Genetica Agraria|
|Data di pubblicazione:||2010|
|Tipologia:||Book Part (author)|
|Appare nelle tipologie:||03 - Contributo in volume|