Eukaryotic organisms have developed a structure, called centromere, able to preserve the integrity of the genome during cell division. A young bull from the Marchigiana breed, with a normal external phenotype, underwent routine cytogenetic analysis to enter the reproduction center. All metaphases analyzed showed an unusual biarmed chromosome of medium size despite a diploid set of chromosomes (2n = 60,XY). FISH analysis excluded a pericentric inversion or a reciprocal translocation, but highlighted a repositioning of the centromere in BTA17. The satellite DNA was still in an acrocentric position. The telomeres were normally present. The primary constriction on the abnormal chromosome was C-band negative. Finally, the absence of a large genomic deletion in the BTA17 pericentromeric region was demonstrated by both array-CGH analysis and SNP array. To our knowledge, this is the first case of centromere repositioning reported in cattle.

Centromere Repositioning in Cattle (Bos taurus) Chromosome 17 / L. De Lorenzi, A. Iannuzzi, E. Rossi, S. Bonacina, P. Parma. - In: CYTOGENETIC AND GENOME RESEARCH. - ISSN 1424-8581. - 151:4(2017), pp. 191-197. [10.1159/000473781]

Centromere Repositioning in Cattle (Bos taurus) Chromosome 17

L. De Lorenzi;BONACINA, STEFANIA;P. Parma
2017

Abstract

Eukaryotic organisms have developed a structure, called centromere, able to preserve the integrity of the genome during cell division. A young bull from the Marchigiana breed, with a normal external phenotype, underwent routine cytogenetic analysis to enter the reproduction center. All metaphases analyzed showed an unusual biarmed chromosome of medium size despite a diploid set of chromosomes (2n = 60,XY). FISH analysis excluded a pericentric inversion or a reciprocal translocation, but highlighted a repositioning of the centromere in BTA17. The satellite DNA was still in an acrocentric position. The telomeres were normally present. The primary constriction on the abnormal chromosome was C-band negative. Finally, the absence of a large genomic deletion in the BTA17 pericentromeric region was demonstrated by both array-CGH analysis and SNP array. To our knowledge, this is the first case of centromere repositioning reported in cattle.
Cattle; Centromere; FISH
Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico
CYTOGENETIC AND GENOME RESEARCH
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/501315
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