A study on presence of antibodies to Trichomonis vaginalis in serum was done on a group of 500 pregnant, asymptomatic Angolan women. A serologic screening, done by ELISA, revealed that 41% of the women had IgG and IgM against the parasite. Analysis of sera by immunoblotting revealed that 94.4% of sera with anti-T. vaginalis IgG class antibodies were reactive against a common immunogenic protein of 115 kDa. The common immunogen was identified as the protozoan α-actinin. All sera recognizing the 115-kDa antigen were reactive against both native and recombinant T. vaginalis α-actinin and nonreactive against human α- actinin. The findings presented in this work offer a new tool for epidemiologic studies and open new perspectives for vaccination.
Identification of Trichomonas vaginalis α-actinin as the most common immunogen recognized by sera of women exposed to the parasite / M.F. Addis, P. Rappelli, A.M.P. De Andrade, F.M. Rita, M.M. Colombo, P. Cappuccinelli, P.L. Fiori. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 0022-1899. - 180:5(1999 Nov), pp. 1727-1730.
|Titolo:||Identification of Trichomonas vaginalis α-actinin as the most common immunogen recognized by sera of women exposed to the parasite|
ADDIS, MARIA FILIPPA (Primo)
|Parole Chiave:||actinin; animals; antibodies, protozoan; antigens, protozoan; blotting, western; electrophoresis, polyacrylamide gel; enzyme-linked immunosorbent assay; female; humans; male; pregnancy; pregnancy complications, parasitic; recombinant proteins; trichomonas vaginitis; trichomonas vaginalis; public health, environmental and occupational health; immunology|
|Settore Scientifico Disciplinare:||Settore BIO/19 - Microbiologia Generale|
Settore BIO/10 - Biochimica
|Data di pubblicazione:||nov-1999|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1086/315095|
|Appare nelle tipologie:||01 - Articolo su periodico|