This paper reports the identification and characterisation of a 60 kDa surface protein antigen (P60) of Mycoplasma capricolum subspecies capricolum (Mcc), and describes its diagnostic application. Genomic localization and presence in P60 of conserved functional domains suggested a structural and functional relationship with the immunodominant antigen P48 of Mycoplasma agalactiae, a basic membrane protein. A rP60-ELISA was developed, and it resulted in a high specificity for Mcc infections after evaluation with 125 goat sera. The comparison with an existent ELISA based on whole Mcc cell lysates showed that the two assays have comparable sensitivities, but the rP60-ELISA has the significant advantage of a greater specificity. Results indicate that P60 is a potential marker of Mcc infection, especially useful in areas where the presence of M. capricolum subspecies capripenumoniae is also reported.

Characterisation of Mycoplasma capricolum P60 surface lipoprotein and its evaluation in a recombinant ELISA / A. Alberti, P. Robino, B. Chessa, S. Rosati, M.F. Addis, P. Mercier, A. Mannelli, T. Cubeddu, M. Profiti, E. Bandino, R. Thiery, M. Pittau. - In: VETERINARY MICROBIOLOGY. - ISSN 0378-1135. - 128:1-2(2008 Apr), pp. 81-89.

Characterisation of Mycoplasma capricolum P60 surface lipoprotein and its evaluation in a recombinant ELISA

M.F. Addis;
2008

Abstract

This paper reports the identification and characterisation of a 60 kDa surface protein antigen (P60) of Mycoplasma capricolum subspecies capricolum (Mcc), and describes its diagnostic application. Genomic localization and presence in P60 of conserved functional domains suggested a structural and functional relationship with the immunodominant antigen P48 of Mycoplasma agalactiae, a basic membrane protein. A rP60-ELISA was developed, and it resulted in a high specificity for Mcc infections after evaluation with 125 goat sera. The comparison with an existent ELISA based on whole Mcc cell lysates showed that the two assays have comparable sensitivities, but the rP60-ELISA has the significant advantage of a greater specificity. Results indicate that P60 is a potential marker of Mcc infection, especially useful in areas where the presence of M. capricolum subspecies capripenumoniae is also reported.
basic membrane proteins; contagious agalactia; elisa; mycoplasma capricolum subspecies capricolum; p60; animals; antibodies, bacterial; bacterial proteins; base sequence; cloning, molecular; enzyme-linked immunosorbent assay; goat diseases; goats; lipoproteins; molecular sequence data; mycoplasma agalactiae; mycoplasma capricolum; pleuropneumonia, contagious; point mutation; rabbits; recombinant fusion proteins; sensitivity and specificity; animal science and zoology; microbiology; veterinary (all)
Settore VET/05 - Malattie Infettive degli Animali Domestici
Settore BIO/19 - Microbiologia Generale
apr-2008
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/492889
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