Cells of the artery wall may accumulate free cholesterol and cholesteryl esters becoming foam cells. Up to 50% of foam cells in human lesions originates from smooth muscle cells (SMCs). Arterial SMCs express the ATP binding cassette (ABC) transporter ABCA1 and, upon cholesterol loading, express macrophage markers and a phagocytic activity. To characterize the role of ABCA1 and HDL3 in this transdifferentiation process, we evaluated the phenotypic changes in SMCs isolated from wild type (WT) and ABCA1 knock out (KO) mice and how HDL3 affects these changes. Cholesterol loading causes the downregulation of the expression of SMC markers including ACTA2, alpha-tropomyosin and myosin heavy chain and increases the expression of macrophage-related genes such as CD68, Mac-2, SRB1, MMPs, ABCG1 and ABCA1. HDL3 treatment in WT cells is able to normalize the expression of ACTA2, while the expression of macrophage-related genes is reduced. On the contrary, the preventive effect of HDL3 is completely lost in ABCA1 KO cells. Interestingly, the presence of HDL3 does not differently affect neutral lipid accumulation in WT or ABCA1 KO cells but stimulates phospholipids removal only in WT cells. ApoAI addition does not reverse the phenotypic changes induced by cholesterol not only in KO but also in WT cells. Moreover, cholesterol loading reduces the expression of myocardin, the master SMC specific-transcriptional coactivator involved in SMC differentiation, by up to 55% (p<0.01 vs respective control) in both cell types. HDL3 normalizes myocardin levels in WT cells while it does not have any effect in ABCA1 KO cells. Similar results are obtained evaluating the levels of miR-143/145, which positively regulate myocardin. The basal expression level of KLF4, a myocardin repressor, is almost double in ABCA1 KO cells compared to WT. After cholesterol loading, KLF4 is slightly reduced in WT cells, while its expression is halved in ABCA1 KO cells. HDL3 restores KLF4 to basal levels in KO cells, but it further reduces them in WT cells. These results indicate that HDL3, modulating the miR143/145-myocardin axis in SMC, prevents the cholesterol-induced gene expression modification regardless of its cholesterol unloading capacity and the presence of ABCA1 is required.
ABCA1 and HDL3 are Required to Modulate Smooth Muscle Cells Transdifferentiation in a Myocardin-miR143/145-Dependent Process / S. Castiglioni, L. Arnaboldi, A. Vettore, L. Calabresi, A. Corsini, S. Bellosta. - In: ARTERIOSCLEROSIS, THROMBOSIS, AND VASCULAR BIOLOGY. - ISSN 1079-5642. - 36:Suppl 1(2016 May). ((Intervento presentato al convegno Arteriosclerosis, Thrombosis and Vascular Biology I Peripheral Vascular Disease Scientific Sessions 2016 tenutosi a Nashville nel 2016.
|Titolo:||ABCA1 and HDL3 are Required to Modulate Smooth Muscle Cells Transdifferentiation in a Myocardin-miR143/145-Dependent Process|
CASTIGLIONI, SILVIA (Primo)
VETTORE, ALESSIO (Secondo)
CORSINI, ALBERTO (Penultimo)
BELLOSTA, STEFANO (Corresponding)
|Parole Chiave:||smooth muscle cells, phenotypic switch, cholesterol, atherosclerosis|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||mag-2016|
|Appare nelle tipologie:||01 - Articolo su periodico|