To evaluate the clinical usefulness of IL-2 in myelodysplastic syndromes (MDS) the in vitro effects of interleukin-2 (IL-2) on blast cell proliferation, clonogenic activity, cytokine release and cell mediated cytotoxicity were examined in 49 MDS patients. Morphological analyses of bone marrow (BM) cytospin preparations showed a significant decrease in the number of blast cells in MDS after incubation with IL-2. Incubation of bone marrow mononuclear cells (BMMNCs) with IL-2 induced a significant increase in the number of CFU-GM in comparison with untreated controls. gamma-IFN and GM-CSF, but not alpha-TNF were found to be released in significant amounts by the BMMNCs cultured with IL-2. No significant differences in the surface phenotypes of fresh lymphocytes were observed between the normal and MDS subjects. After incubation with IL-2, we observed a significant increase in the number of CD3-/CD56+ cells in both normal and MDS subjects. Peripheral blood (PB) and BM NK activity against K562 was significantly greater in MDS after stimulation with IL-2. These data suggest the clinical usefulness of IL-2 in a large subgroup of patients as it may reduce the percentage of blasts and increase clonogenic capacity and cell-mediated cytotoxicity.

In vitro effects of IL-2 on NK activity, clonogenic potential, blast cell proliferation and cytokine release of MDS bone marrow patients / A. Cortelezzi, B. Sarina, C. Cattaneo, M. Pomati, I. Silvestris, D. Soligo, R. Calori, M. Di Stefano, C. Hu, M. Monza, L. Radaelli, A.T. Maiolo. - In: LEUKEMIA. - ISSN 0887-6924. - 10:7(1996 Jul), pp. 1181-1189.

In vitro effects of IL-2 on NK activity, clonogenic potential, blast cell proliferation and cytokine release of MDS bone marrow patients

A. Cortelezzi
Primo
;
M. Pomati;I. Silvestris;D. Soligo;A.T. Maiolo
Ultimo
1996

Abstract

To evaluate the clinical usefulness of IL-2 in myelodysplastic syndromes (MDS) the in vitro effects of interleukin-2 (IL-2) on blast cell proliferation, clonogenic activity, cytokine release and cell mediated cytotoxicity were examined in 49 MDS patients. Morphological analyses of bone marrow (BM) cytospin preparations showed a significant decrease in the number of blast cells in MDS after incubation with IL-2. Incubation of bone marrow mononuclear cells (BMMNCs) with IL-2 induced a significant increase in the number of CFU-GM in comparison with untreated controls. gamma-IFN and GM-CSF, but not alpha-TNF were found to be released in significant amounts by the BMMNCs cultured with IL-2. No significant differences in the surface phenotypes of fresh lymphocytes were observed between the normal and MDS subjects. After incubation with IL-2, we observed a significant increase in the number of CD3-/CD56+ cells in both normal and MDS subjects. Peripheral blood (PB) and BM NK activity against K562 was significantly greater in MDS after stimulation with IL-2. These data suggest the clinical usefulness of IL-2 in a large subgroup of patients as it may reduce the percentage of blasts and increase clonogenic capacity and cell-mediated cytotoxicity.
Clonogenic activity; Cytokines; Interleukin-2; Myelodysplastic syndromes
Settore MED/15 - Malattie del Sangue
lug-1996
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/47270
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