Natural antisense transcripts, because of their potential to form double-stranded RNA (dsRNA) molecules, recently emerged as a mechanism acting on eukaryotic gene regulation at multiple levels. CHRNA3 and CHRNA5, coding for alpha3 and alpha5 subunits of the neuronal nicotinic acetylcholine receptor, have been reported to overlap at their 3'ends in human and bovine genomes. The general goal of this PhD project was the structural and functional study of this overlap. A fine characterization of human CHRNA3 and CHRNA5 alternative transcripts allowed a more precise definition of the overlap extent and highlighted a complex web of possible sense-antisense interactions among overlapping mRNAs. An RNase protection-based approach was used to detect in vivo RNA-RNA duplexes of CHRNA3 and CHRNA5 transcripts, which resulted to be present in human but not in bovine cells. Furthermore, levels of overlapping transcripts were determined by real-time RT-PCR both in humans and in cattle. Finally, some possible functional consequences of this overlap were explored.
|Titolo:||Alpha3 and alpha5 neuronal nicotinic receptor subunit genes : a case of tail-to-tail overlap in humans|
|Supervisori e coordinatori interni:||ZANETTI, GIULIANA|
|Data di pubblicazione:||10-gen-2005|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
Settore BIO/10 - Biochimica
|Citazione:||Alpha3 and alpha5 neuronal nicotinic receptor subunit genes : a case of tail-to-tail overlap in humans ; G. Zanetti, M. Malcovati. - Milano : Università degli studi di Milano. DIPARTIMENTO DI BIOLOGIA E GENETICA PER LE SCIENZE MEDICHE, 2005 Jan 10. ((17. ciclo, Anno Accademico 2003/2004.|
|Appare nelle tipologie:||13 - Tesi di dottorato discussa entro ottobre 2010|