The usual rate of actin polymerization is increased if one starts from action nuclei. We have noticed that, using α-actinin crosslinked actin nuclei, the initial net elongation rate is further enhanced. Also initial net depolymerization rates of α-actinin crosslinked F-actin samples are higher than those of controls. These results should imply that α-actinin increases the filament end concentration of actin samples. The experiments with barbed and blocking substances (cytochalasin D and gelsolin-actin complex) confirmed such an increase. We have shown that: (1) α-actinin does not significantly influence actin polymerization over all; (2) α-actinin inhibits the recovery of the filament size in F-actin samples after sonication; and (3) the influence of α-actinin on actin filament end concentration is counteracted by tropomyosin. Therefore, we suggest that, upon filament shearing, α-actinin crosslinking inhibits the annealing of short actin polymers into longer filaments.
alpha-Actinin increases actin filament end concentration by inhibiting annealing / R. Colombo, I. Dalle Donne, A. Milzani. - In: JOURNAL OF MOLECULAR BIOLOGY. - ISSN 0022-2836. - 230:4(1993), pp. 1151-1158. [10.1006/jmbi.1993.1232]
alpha-Actinin increases actin filament end concentration by inhibiting annealing
I. Dalle DonneSecondo
;A. MilzaniUltimo
1993
Abstract
The usual rate of actin polymerization is increased if one starts from action nuclei. We have noticed that, using α-actinin crosslinked actin nuclei, the initial net elongation rate is further enhanced. Also initial net depolymerization rates of α-actinin crosslinked F-actin samples are higher than those of controls. These results should imply that α-actinin increases the filament end concentration of actin samples. The experiments with barbed and blocking substances (cytochalasin D and gelsolin-actin complex) confirmed such an increase. We have shown that: (1) α-actinin does not significantly influence actin polymerization over all; (2) α-actinin inhibits the recovery of the filament size in F-actin samples after sonication; and (3) the influence of α-actinin on actin filament end concentration is counteracted by tropomyosin. Therefore, we suggest that, upon filament shearing, α-actinin crosslinking inhibits the annealing of short actin polymers into longer filaments.File | Dimensione | Formato | |
---|---|---|---|
Colombo 1993 JMB.pdf
accesso riservato
Tipologia:
Publisher's version/PDF
Dimensione
444.1 kB
Formato
Adobe PDF
|
444.1 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.