Background: Paraoxonase 1 (PON1) gene polymorphisms and polyphenols intake have been reported independently associated to lipid profile and susceptibility to atherosclerosis and cardiovascular disease. However, the interaction between these factors remains to be investigated. We performed an observational nutrigenetic study to examine whether the interaction between polyphenols and anthocyanins intake and PON1 genetic variants can modulate biomarkers of cardiovascular health in an Italian healthy population. Methods: We recruited 443 healthy volunteers who participated in the EC funded ATHENA project (AnThocyanin and polyphenols bioactive for Health Enhancement through Nutritional Advancement). Data collection included detailed demographic, clinical, dietary, lifestyle, biochemical and genetic data. Polyphenols and anthocyanins intake was measured by 24h dietary recall repeated three times a year in order to get seasonal variations. We tested the interaction between 18 independent tagging SNPs in PON1 gene and polyphenols intake on HDL, LDL, cholesterol, triglycerides and atherogenic index of plasma. Results: Without considering the genetic background, we could not observe significant differences in the lipid profile between high and low polyphenols and anthocyanins intake. Using a nutrigenetic approach, we identified protective genotypes in four independent polymorphisms that, at Bonferroni level (p≤0.0028), present a significant association with increased HDL level under high polyphenols and anthocyanins intake, compared to risk genotypes (rs854549, Beta=4.7 per C allele; rs854552, Beta=5.6 per C allele; rs854571, Beta=3.92 per T allele; rs854572, Beta=3.94 per C allele). Conclusions: We highlight the protective role of genetic variants in PON1 towards cardiovascular risk under high polyphenols and anthocyanins consumption. PON1 variants could represent novel biomarkers to stratify individuals who might benefit from targeted dietary recommendation for health promotion and strategies of preventive medicine.

Interaction between polyphenols intake and PON1 gene variants on markers of cardiovascular disease : A nutrigenetic observational study / F. Rizzi, C. Conti, E. Dogliotti, A. Terranegra, E. Salvi, D. Braga, F. Ricca, S. Lupoli, A. Mingione, F. Pivari, C. Brasacchio, M. Barcella, M. Chittani, F. D'Avila, M. Turiel, M. Lazzaroni, L. Soldati, D. Cusi, C. Barlassina. - In: JOURNAL OF TRANSLATIONAL MEDICINE. - ISSN 1479-5876. - 14:1(2016). [10.1186/s12967-016-0941-6]

Interaction between polyphenols intake and PON1 gene variants on markers of cardiovascular disease : A nutrigenetic observational study

F. Rizzi
Primo
;
C. Conti
Secondo
;
E. Dogliotti;A. Terranegra;E. Salvi;D. Braga;S. Lupoli;A. Mingione;F. Pivari;C. Brasacchio;M. Barcella;M. Chittani;F. D'Avila;M. Turiel;L. Soldati;D. Cusi
Penultimo
;
2016

Abstract

Background: Paraoxonase 1 (PON1) gene polymorphisms and polyphenols intake have been reported independently associated to lipid profile and susceptibility to atherosclerosis and cardiovascular disease. However, the interaction between these factors remains to be investigated. We performed an observational nutrigenetic study to examine whether the interaction between polyphenols and anthocyanins intake and PON1 genetic variants can modulate biomarkers of cardiovascular health in an Italian healthy population. Methods: We recruited 443 healthy volunteers who participated in the EC funded ATHENA project (AnThocyanin and polyphenols bioactive for Health Enhancement through Nutritional Advancement). Data collection included detailed demographic, clinical, dietary, lifestyle, biochemical and genetic data. Polyphenols and anthocyanins intake was measured by 24h dietary recall repeated three times a year in order to get seasonal variations. We tested the interaction between 18 independent tagging SNPs in PON1 gene and polyphenols intake on HDL, LDL, cholesterol, triglycerides and atherogenic index of plasma. Results: Without considering the genetic background, we could not observe significant differences in the lipid profile between high and low polyphenols and anthocyanins intake. Using a nutrigenetic approach, we identified protective genotypes in four independent polymorphisms that, at Bonferroni level (p≤0.0028), present a significant association with increased HDL level under high polyphenols and anthocyanins intake, compared to risk genotypes (rs854549, Beta=4.7 per C allele; rs854552, Beta=5.6 per C allele; rs854571, Beta=3.92 per T allele; rs854572, Beta=3.94 per C allele). Conclusions: We highlight the protective role of genetic variants in PON1 towards cardiovascular risk under high polyphenols and anthocyanins consumption. PON1 variants could represent novel biomarkers to stratify individuals who might benefit from targeted dietary recommendation for health promotion and strategies of preventive medicine.
anthocyanins; antioxidants; gene diet interaction; genetic variants; hdl; lipid profile; nutrigenomics; polyphenols; pon1 gene; biochemistry, genetics and molecular biology (all); medicine (all)
Settore BIO/18 - Genetica
Settore MED/14 - Nefrologia
Article (author)
File in questo prodotto:
File Dimensione Formato  
RizziBarlassina_InteractionPolyphenols_JournalTranslatationalMedicine_2016.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 1.1 MB
Formato Adobe PDF
1.1 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/446624
Citazioni
  • ???jsp.display-item.citation.pmc??? 14
  • Scopus 27
  • ???jsp.display-item.citation.isi??? 27
social impact