TheGENOMESUNCOUPLED1(GUN1)genehasbeenreportedtoencodeachloroplast-localizedpentatricopeptide-repeatprotein,whichactstointegratemultipleindicatorsofplastiddevelopmentalstageandalteredplastidfunction,aspartofchloroplast-to-nucleusretrogradecommunication.However,themolecularmechanismsunderlyingsignalintegrationbyGUN1haveremainedelusive,upuntiltherecentidentificationofasetofGUN1-interactingproteins,byco-immunoprecipitationandmass-spectrometricanalyses,aswellasprotein–proteininteractionassays.Here,wereviewthemolecularfunctionsofthedifferentGUN1partnersandproposeamajorroleforGUN1ascoordinatorofchloroplasttranslation,proteinimport,andproteindegradation.Thisregulatoryroleisimplementedthroughproteinsthat,inmostcases,arepartofmultimericproteincomplexesandwhoseprecisefunctionsvarydependingontheirassociationstates.Withinthisframework,GUN1mayactasaplatformtopromotespecificfunctionsbybringingtheinteractingenzymesintocloseproximitywiththeirsubstrates,ormayinhibitprocessesbysequesteringparticularpoolsofspecificinteractors.Furthermore,theinteractionsofGUN1withenzymesofthetetrapyrrolebiosynthesis(TPB)pathwaysupporttheinvolvementoftetrapyrrolesassignalingmoleculesinretrogradecommunication.
GUN1, a Jack-Of-All-Trades in Chloroplast Protein Homeostasis and Signaling / M. Colombo, L. Tadini, C. Peracchio, R. Ferrari, P. Pesaresi. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - 7:(2016 Sep 22), pp. 1427.1-1427.14. [10.3389/fpls.2016.01427]
GUN1, a Jack-Of-All-Trades in Chloroplast Protein Homeostasis and Signaling
L. TadiniSecondo
;C. Peracchio;R. FerrariPenultimo
;P. PesaresiUltimo
2016
Abstract
TheGENOMESUNCOUPLED1(GUN1)genehasbeenreportedtoencodeachloroplast-localizedpentatricopeptide-repeatprotein,whichactstointegratemultipleindicatorsofplastiddevelopmentalstageandalteredplastidfunction,aspartofchloroplast-to-nucleusretrogradecommunication.However,themolecularmechanismsunderlyingsignalintegrationbyGUN1haveremainedelusive,upuntiltherecentidentificationofasetofGUN1-interactingproteins,byco-immunoprecipitationandmass-spectrometricanalyses,aswellasprotein–proteininteractionassays.Here,wereviewthemolecularfunctionsofthedifferentGUN1partnersandproposeamajorroleforGUN1ascoordinatorofchloroplasttranslation,proteinimport,andproteindegradation.Thisregulatoryroleisimplementedthroughproteinsthat,inmostcases,arepartofmultimericproteincomplexesandwhoseprecisefunctionsvarydependingontheirassociationstates.Withinthisframework,GUN1mayactasaplatformtopromotespecificfunctionsbybringingtheinteractingenzymesintocloseproximitywiththeirsubstrates,ormayinhibitprocessesbysequesteringparticularpoolsofspecificinteractors.Furthermore,theinteractionsofGUN1withenzymesofthetetrapyrrolebiosynthesis(TPB)pathwaysupporttheinvolvementoftetrapyrrolesassignalingmoleculesinretrogradecommunication.
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