BACKGROUND: Skin is constantly in contact with different pathogens, which are present in the environment. The hair follicle is particularly susceptible to this microbial invasion as it offers an easy way of access for microorganisms; for this reason it is equipped with defence mechanisms to avoid frequent infections. OBJECTIVES: To analyse the expression pattern of four different members of the toll-like receptor (TLR) family in murine hair follicles and to evaluate the effects of their activation by their specific microbiota-derived agonists, in terms of production of the antimicrobial peptide beta-defensin 2 (DEFB2). METHODS: TLR and DEFB2 protein expression was investigated by immunohistochemistry on murine skin samples. RESULTS: Murine hair follicle expresses TLR2, TLR4 and TLR5; agonists of TLR2, TLR4 and TLR5 but not of TLR9 induced DEFB2 production in this compartment. The strongest DEFB2 expression was observed following TLR4 activation by lipopolysaccharide. CONCLUSIONS: Our data show that the hair follicle is equipped with TLR2, TLR4 and TLR5, and that these receptors are able to respond to microbial stimuli inducing the production of DEFB2 by epithelial cells. This immune response might be important in preserving the skin from microorganism infections.

Toll-like receptor agonists regulate β-defensin 2 release in hair follicle / S. Selleri, F. Arnaboldi, M. Palazzo, S. Gariboldi, L. Zanobbio, E. Opizzi, Y.F. Shirai, A. Balsari, C. Rumio. - In: BRITISH JOURNAL OF DERMATOLOGY. - ISSN 0007-0963. - 156:6(2007 Jun), pp. 1172-1177.

Toll-like receptor agonists regulate β-defensin 2 release in hair follicle

S. Selleri;F. Arnaboldi;M. Palazzo;S. Gariboldi;L. Zanobbio;E. Opizzi;Y.F. Shirai;A. Balsari;C. Rumio
2007

Abstract

BACKGROUND: Skin is constantly in contact with different pathogens, which are present in the environment. The hair follicle is particularly susceptible to this microbial invasion as it offers an easy way of access for microorganisms; for this reason it is equipped with defence mechanisms to avoid frequent infections. OBJECTIVES: To analyse the expression pattern of four different members of the toll-like receptor (TLR) family in murine hair follicles and to evaluate the effects of their activation by their specific microbiota-derived agonists, in terms of production of the antimicrobial peptide beta-defensin 2 (DEFB2). METHODS: TLR and DEFB2 protein expression was investigated by immunohistochemistry on murine skin samples. RESULTS: Murine hair follicle expresses TLR2, TLR4 and TLR5; agonists of TLR2, TLR4 and TLR5 but not of TLR9 induced DEFB2 production in this compartment. The strongest DEFB2 expression was observed following TLR4 activation by lipopolysaccharide. CONCLUSIONS: Our data show that the hair follicle is equipped with TLR2, TLR4 and TLR5, and that these receptors are able to respond to microbial stimuli inducing the production of DEFB2 by epithelial cells. This immune response might be important in preserving the skin from microorganism infections.
β-defensin 2 ; hair follicle ; mouse ; skin ; toll-like receptor
Settore MED/04 - Patologia Generale
Settore BIO/16 - Anatomia Umana
giu-2007
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/43593
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