Aim: Adipose-derived stem cells (ASCs) have been deeply characterized for their usefulness in musculoskeletal tissue regeneration; recently, other mesenchymal stem cell (MSC) sources have also been proposed. This study compares for the first time human tendon stem/progenitor cells isolated from hamstring tendons with human ASCs. Materials & Methods: Human TSPCs and ASCs were isolated from hamstring tendon portions and adipose tissue of healthy donors undergoing ACL reconstruction or liposuction, respectively (n = 7). Clonogenic ability, immunophenotype and multi-differentiation potential were assessed and compared. Results: Both populations showed similar proliferation and clonogenic ability and expressed embryonic stem cell genes and MSC surface markers. Tendon stem/progenitor cells showed lower adipogenic and osteogenic ability, but after the chondrogenic differentiation, they produced more abundant glycosaminoglycans and expressed higher levels of aggrecan with regards to ASCs. The tenogenic induction with BMP-12 upregulated SCX and DCN gene expression in both populations. Conclusion: Our results demonstrate that waste hamstring tendon fragments could represent a convenient MSC source for musculoskeletal regenerative medicine.
Multidifferentiation potential of human mesenchymal stem cells from adipose tissue and hamstring tendons for musculoskeletal cell-based therapy / D. Stanco, M. Viganò, C. Perucca Orfei, A. Di Giancamillo, G.M. Peretti, L. Lanfranchi, L. De Girolamo. - In: REGENERATIVE MEDICINE. - ISSN 1746-0751. - 10:6(2015), pp. 729-743.
|Titolo:||Multidifferentiation potential of human mesenchymal stem cells from adipose tissue and hamstring tendons for musculoskeletal cell-based therapy|
STANCO, DEBORAH (Primo)
|Parole Chiave:||adipose-derived stem cells; bone morphogenetic protein-12; multidifferentiation potential; musculoskeletal pathologies; regenerative medicine; tendon; tendon stem progenitor cells; tenogenic differentiation; Adipocytes; Adipose Tissue; Bone Morphogenetic Proteins; Cell Differentiation; Cell Proliferation; Cell Separation; Cell- and Tissue-Based Therapy; Chondrocytes; Fibroblasts; Flow Cytometry; Gene Expression Regulation; Growth Differentiation Factors; Humans; Immunophenotyping; Mesenchymal Stromal Cells; Muscle, Skeletal; Osteogenesis; Regeneration; Regenerative Medicine; Stem Cell Transplantation; Tendons; Up-Regulation; Biomedical Engineering; Embryology; Medicine (all)|
|Settore Scientifico Disciplinare:||Settore MED/33 - Malattie Apparato Locomotore|
Settore VET/01 - Anatomia degli Animali Domestici
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.2217/rme.14.92|
|Appare nelle tipologie:||01 - Articolo su periodico|