Activation of the receptor tyrosine kinase ErbB4 leads to various cellular responses such as proliferation, survival, differentiation, and chemotaxis. Two pairs of naturally occurring ErbB4 isoforms differing in their juxtamembrane (JMa/JMb) and C termini (cyt1/cyt2) have been described. To examine the role of ErbB4 in neuron migration, we cloned and stably transfected each of the four ErbB4 isoforms in ST14A cells (a neural progenitor cell line derived from the striatum of embryonic day 14 rats) endogenously expressing the other members of the ErbB family: ErbB1, ErbB2, and ErbB3. Using immunoprecipitation assays, we showed that the neuregulin-1beta1 (NRG1beta1) stimulus induced ErbB4 tyrosine phosphorylation and phosphatidylinositol 3-kinase (PI3K) recruitment and activation (as demonstrated by Akt phosphorylation) either directly (ErbB4 cyt1 isoform) or indirectly (ErbB4 cyt2 isoform). We examined the ability of the four ErbB4 isoforms to induce chemotaxis and cell proliferation in response to NRG1beta1 stimulation. Using migration assays, we observed that only ErbB4-expressing cells stimulated with NRG1beta1 showed a significant increase in migration, whereas the growth rate remained unchanged. Additional assays showed that inhibition of PI3K (but not of phospholipase Cgamma) dramatically reduced migratory activity. Our data show that ErbB4 signaling via PI3K activation plays a fundamental role in controlling NRG1beta1-induced migration.
ErbB4 expression in neural progenitor cells (ST14A) is necessary to mediate neuregulin-1beta1-induced migration / G. Gambarotta, D. Garzotto, E. Destro, B. Mautino, C. Giampietro, S. Cutrupi, C. Dati, E. Cattaneo, A. Fasolo, I. Perroteau. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 279:47(2004 Nov 19), pp. 48808-48816.
|Titolo:||ErbB4 expression in neural progenitor cells (ST14A) is necessary to mediate neuregulin-1beta1-induced migration|
|Parole Chiave:||animals; blotting, western; cos cells; cell differentiation; cell membrane; cell movement; cell proliferation; cells, cultured; chemotaxis; cloning, molecular; dna, complementary; dimerization; enzyme activation; immunoprecipitation; models, biological; molecular sequence data; neuregulin-1; neurons; phosphatidylinositol 3-kinases; phospholipase c gamma; phosphorylation; plasmids; protein binding; protein isoforms; protein structure, tertiary; rats; receptor, epidermal growth factor; receptor, erbb-3; receptor, erbb-4; reverse transcriptase polymerase chain reaction; sequence analysis, dna; signal transduction; stem cells; time factors; transfection; type c phospholipases|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
Settore BIO/14 - Farmacologia
Settore BIO/06 - Anatomia Comparata e Citologia
|Data di pubblicazione:||19-nov-2004|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1074/jbc.M408374200|
|Appare nelle tipologie:||01 - Articolo su periodico|