Background: Ewing's sarcoma cell lines may represent a good in vitro model for the understanding of tumor biology in this heterogeneous group of diseases. In the present study, we report the establishment and characterization of a primary Ewing's sarcoma cell line (LDS-Falck 01). Materials and Methods: LDS-Falck 01 was generated from a malignant pleural effusion of a patient with metastatic peripheral primitive neuroectodermal tumor arising from the chest wall. Extensive characterization of the cells was accomplished using immunocytochemical, RT-PCR and cytogenetic studies. Results: In vitro LDS-Falck 01 cells had both anchorage-dependent and -independent growth patterns. Immunocytochemical studies showed that cells were PAS-, vimentin-, CD99- and NSE-positive, EGFR- and CD117-negative. Cytogenetic analysis revealed a complex hyperdiploid katyotype with multiple chromosomal aberrations including an unbalanced translocation t(11;22)(q24,-q12). The EWSIFLI1 chimeric transcript type I was detected. Conclusion: This cell line may represent a valid tool for investigating the biomolecular characteristics of this group of neoplasms and their sensitivity to therapeutic agents.
Establishment and characterization of a new Ewing's sarcoma cell line from a malignant pleural effusion / R. Schiavo, C. Tullio, M. La Grotteria, I.C. Andreotti, B. Scarpati, L. Romiti, F. Bozzi, P. Pedrazzoli, S. Siena. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - 27:5A(2007), pp. 3273-3278.
Establishment and characterization of a new Ewing's sarcoma cell line from a malignant pleural effusion
S. SienaUltimo
2007
Abstract
Background: Ewing's sarcoma cell lines may represent a good in vitro model for the understanding of tumor biology in this heterogeneous group of diseases. In the present study, we report the establishment and characterization of a primary Ewing's sarcoma cell line (LDS-Falck 01). Materials and Methods: LDS-Falck 01 was generated from a malignant pleural effusion of a patient with metastatic peripheral primitive neuroectodermal tumor arising from the chest wall. Extensive characterization of the cells was accomplished using immunocytochemical, RT-PCR and cytogenetic studies. Results: In vitro LDS-Falck 01 cells had both anchorage-dependent and -independent growth patterns. Immunocytochemical studies showed that cells were PAS-, vimentin-, CD99- and NSE-positive, EGFR- and CD117-negative. Cytogenetic analysis revealed a complex hyperdiploid katyotype with multiple chromosomal aberrations including an unbalanced translocation t(11;22)(q24,-q12). The EWSIFLI1 chimeric transcript type I was detected. Conclusion: This cell line may represent a valid tool for investigating the biomolecular characteristics of this group of neoplasms and their sensitivity to therapeutic agents.File | Dimensione | Formato | |
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